Genomic chart guiding embryonic stem cell cardiopoiesis

Genome Biology
Randolph S FaustinoAndre Terzic

Abstract

Embryonic stem cells possess a pluripotent transcriptional background with the developmental capacity for distinct cell fates. Simultaneous expression of genetic elements for multiple outcomes obscures cascades relevant to specific cell phenotypes. To map molecular patterns critical to cardiogenesis, we interrogated gene expression in stem cells undergoing guided differentiation, and defined a genomic paradigm responsible for confinement of pluripotency. Functional annotation analysis of the transcriptome of differentiating embryonic stem cells exposed downregulated components of DNA replication, recombination and repair machinery, cell cycling, cancer mechanisms, and RNA post-translational modifications. Concomitantly, cardiovascular development, cell-to-cell signaling, cell development and cell movement were upregulated. These simultaneous gene ontology rearrangements engaged a repertoire switch that specified lineage development. Bioinformatic integration of genomic and gene ontology data further unmasked canonical signaling cascades prioritized within discrete phases of cardiopoiesis. Examination of gene relationships revealed a non-stochastic network anchored by integrin, WNT/beta-catenin, transforming growth factor beta a...Continue Reading

References

Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·J L CollE D Adamson
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·K YoshidaT Kishimoto
Nov 6, 1998·Science·J A ThomsonJ M Jones
Jul 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·M K Kerr, G A Churchill
Jan 24, 2002·Nature·Piero Anversa, Bernardo Nadal-Ginard
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 14, 2002·Cardiovascular Research·Marielle E van GijnW Matthijs Blankesteijn
Oct 11, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Atta BehfarMichel Pucéat
Nov 6, 2002·Genetic Engineering·Shaw-Yung ShaiRobert S Ross
Nov 12, 2002·Nature Biotechnology·Ren-He XuJames A Thomson
Feb 26, 2003·Circulation Research·Carmen Perez-TerzicMichel Pucéat
Jul 5, 2003·Genes & Development·Stephane D VincentElizabeth J Robertson
Jan 22, 2004·Nature Reviews. Genetics·Albert-László Barabási, Zoltán N Oltvai
Mar 12, 2004·Cancer Letters·Manfred Schwab
May 4, 2004·Trends in Cardiovascular Medicine·Ann Foley, Mark Mercola
Jul 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Denice M HodgsonAndre Terzic
Nov 3, 2004·Nature Reviews. Genetics·Dov Zipori
Feb 17, 2005·Molecular and Cellular Biology·Rachael M EastonMorris J Birnbaum
Mar 15, 2005·The Journal of Clinical Investigation·Stefanie DimmelerMichael D Schneider
Jun 21, 2005·Annals of the New York Academy of Sciences·Atta BehfarAndre Terzic
Jul 5, 2005·Journal of Molecular and Cellular Cardiology·S VanderveldeM C Harmsen
Jul 9, 2005·Nature Biotechnology·Michael A Laflamme, Charles E Murry
Sep 6, 2005·Science·P CarninciUNKNOWN RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Oct 18, 2005·Nature Reviews. Molecular Cell Biology·Michele Boiani, Hans R Schöler
Oct 29, 2005·Journal of Cell Science·Réka Albert
Nov 24, 2005·Nature Reviews. Genetics·Margaret BuckinghamStéphane Zaffran
Dec 22, 2005·Nature Reviews. Genetics·Alfonso Martinez Arias, Penelope Hayward
Feb 28, 2006·Nature Clinical Practice. Cardiovascular Medicine·Atsushi SuzukiJuan Carlos Izpisúa Belmonte
Feb 28, 2006·Nature Clinical Practice. Cardiovascular Medicine·Atta Behfar, Andre Terzic
Apr 26, 2006·Circulation·Brian DeBoschAnthony J Muslin
May 16, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Yu ChenJames P Morgan
May 30, 2006·Developmental Biology·José Rivera-Feliciano, Clifford J Tabin
Jun 2, 2006·Molecular Systems Biology·Peter Uetz, Igor Stagljar

❮ Previous
Next ❯

Citations

Jun 1, 2008·Journal of Cardiovascular Translational Research·Randolph S Faustino, Andre Terzic
Jun 1, 2008·Journal of Cardiovascular Translational Research·Jozef BartunekAndre Terzic
Sep 16, 2010·Journal of Cardiovascular Translational Research·Anca ChiriacTimothy J Nelson
Dec 19, 2012·Journal of Cardiovascular Translational Research·Clifford D L FolmesAndre Terzic
Apr 30, 2011·Cardiovascular Research·Silke R Sperling
Aug 17, 2012·Circulation. Cardiovascular Genetics·D Kent Arrell, Andre Terzic
Apr 13, 2011·PloS One·Alberto MirandaAlfonso Gutierrez-Adan
Jun 7, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·A MirandaA Gutierrez-Adan
Feb 3, 2009·Stem Cell Reviews and Reports·Maritza MayorgaMarc Penn
May 14, 2008·Nature Reviews. Genetics·Seung Yon RheeSorin Draghici
Mar 30, 2016·Trends in Cardiovascular Medicine·Andre Terzic, Atta Behfar
Mar 11, 2009·Differentiation; Research in Biological Diversity·Timothy J NelsonAndre Terzic
Oct 7, 2008·Journal of Molecular and Cellular Cardiology·Atta BehfarAndre Terzic
Feb 18, 2010·Clinical and Translational Science·Timothy J NelsonYasuhiro Ikeda
Aug 20, 2009·Clinical and Translational Science·Randolph S FaustinoAndre Terzic
Aug 2, 2008·Stem Cells·Satsuki YamadaAndre Terzic
Mar 29, 2008·Stem Cells·Timothy J NelsonAndre Terzic
May 1, 2008·Clinical and Translational Science·Atta Behfar, Andre Terzic
Jan 5, 2011·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·D Adam YoungKaren L Christman
Mar 7, 2013·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Russell A GouldJonathan T Butcher
Jan 5, 2010·Journal of Molecular and Cellular Cardiology·Susan ChungPetras P Dzeja
Jul 17, 2015·BMC Systems Biology·Randolph S FaustinoCarmen Perez-Terzic
Mar 23, 2012·Prion·Marilene H Lopes, Tiago G Santos
Dec 21, 2013·Journal of the American College of Cardiology·Jozef BartunekAndre Terzic
Oct 23, 2008·Clinical Pharmacology and Therapeutics·R S Faustino, A Terzic
Feb 13, 2009·Clinical Pharmacology and Therapeutics·J BartunekA Terzic
Jun 3, 2016·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Y LvL Zhang
Jan 15, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Mauro C X PintoRodrigo R Resende
Feb 1, 2014·Circulation Research·Sharon Israeli-RosenbergRobert S Ross
Sep 17, 2013·Circulation. Cardiovascular Genetics·Almudena Martinez-FernandezTimothy J Nelson
Nov 6, 2018·Canadian Journal of Physiology and Pharmacology·Claudia C PrestonRandolph S Faustino
May 11, 2020·Molecular and Cellular Biochemistry·Hojjatollah NazariMohammad Taghi Joghataei
Jun 16, 2018·Experimental and Therapeutic Medicine·Yang LvHaiping Wang

❮ Previous
Next ❯

Datasets Mentioned

BETA
AA500897
GSE6689

Methods Mentioned

BETA
scanning electron microscopy
Electron microscopy
chip
PCR
dissection

Software Mentioned

Metamorph
Expression Analysis Systematic Explorer
Genespring GX
QT
Ingenuity
Vcl
Affymetrix
EASE Database for Annotation , Visualization , and Integrated ...
Cytoscape
Ingenuity Pathways Analysis

Related Concepts

Related Feeds

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Cancer Epigenetics & Methyl-CpG (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. Here is the latest research on cancer epigenetics and methyl-CpG binding proteins including ZBTB38.

Cancer Epigenetics

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Actin, Myosin & Cell Movement

Contractile forces generated by the actin-myosin cytoskeleton are critical for morphogenesis, but the cellular and molecular mechanisms of contraction have been elusive for many cell shape changes and movements. Here is the latest research on the roles of actin and myosin in cell movement.

Cancer Epigenetics and Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cardiac Conduction System

The cardiac conduction system is a specialized tract of myocardial cells responsible for maintaining normal cardiac rhythm. Discover the latest research on the cardiac conduction system here.