Genomic cloning and expression of three murine UDP-galactose: beta-N-acetylglucosamine beta1,3-galactosyltransferase genes.

The Journal of Biological Chemistry
T HennetEric G Berger

Abstract

Based on the detection of expressed sequence tags that are similar to known galactosyltransferase sequences, we have isolated three novel UDP-galactose:beta-N-acetylglucosamine beta1, 3-galactosyltransferase (beta3GalT) genes from a mouse genomic library. The three genes, named beta3GalT-I, -II, and -III, encode type II transmembrane proteins of 326, 422, and 331 amino acids, respectively. The three proteins constitute a distinct subfamily as they do not share any sequence identity with other eucaryotic galactosyltransferases. Also, the entire protein-coding region of the three beta3GalT genes was contained in a single exon, which contrasts with the genomic organization of the beta1,4- and alpha1, 3-galactosyltransferase genes. The three beta3GalT genes were mainly expressed in brain tissue. The expression of the full-length murine genes as recombinant baculoviruses in insect cells revealed that the beta3GalT enzymes share the same acceptor specificity for beta-linked GlcNAc, although they differ in their Km for this acceptor and the donor UDP-Gal. The identification of beta3GalT genes emphasizes the structural diversity present in the galactosyltransferase gene family.

References

Apr 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B A FendersonS Hakomori
May 17, 1990·Nature·F YamamotoS Hakomori
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N L ShaperG F Hollis
Jan 1, 1994·Methods in Enzymology·G S Jacob, P Scudder
Feb 1, 1994·Nature Genetics·S F AltschulJ C Wootton
Feb 1, 1993·Glycobiology·K Drickamer
Jun 15, 1996·European Journal of Biochemistry·N KoyamaN Taniguchi
Dec 1, 1995·Glycobiology·A Harduin-LepersP Delannoy

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Citations

Apr 12, 2002·Chembiochem : a European Journal of Chemical Biology·T BülterL Elling
Dec 2, 1999·Biochimica Et Biophysica Acta·I Brockhausen
Dec 16, 1998·Ryōikibetsu shōkōgun shirīzu·T Tanaka, J Ueki
Jan 11, 2002·European Journal of Biochemistry·Martine MalissardThierry Hennet
Apr 16, 2008·The Journal of Biological Chemistry·Alison V NairnKelley W Moremen
Dec 17, 2008·Molecular and Cellular Biology·Belinda ScheggThierry Hennet
Apr 5, 2003·Annual Review of Biochemistry·John B Lowe, Jamey D Marth
Jan 22, 2000·The Journal of Clinical Investigation·T ImbachT Hennet
Nov 19, 2008·BMC Developmental Biology·Franziska BiellmannThierry Hennet
Apr 20, 2001·Biological Chemistry·C AlbachB Schmitz
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·T ImbachT Hennet
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·D ZhouT Hennet
Nov 23, 2006·Methods in Enzymology·Akira TogayachiHisashi Narimatsu
Feb 7, 2019·Physiological Reviews·Robyn MeechPeter I Mackenzie
Jul 20, 2002·The Journal of Biological Chemistry·Reto MüllerThierry Hennet
Jul 20, 2002·The Journal of Biological Chemistry·Tilo SchwientekHenrik Clausen
Nov 26, 1999·Biochimica Et Biophysica Acta·E G Berger

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