Genomic cloning of 18 kDa oleosin and detection of triacylglycerols and oleosin isoforms in maturing rice and postgerminative seedlings

Journal of Biochemistry
L S WuJ T Tzen

Abstract

Oleosins are hydrophobic proteins localized abundantly in the oil bodies of plant seeds. Two distinct oleosin isoforms of molecular masses 18 and 16 kDa are present in rice oil bodies. These isoforms were found in similar ratio in rice embryos and aleurone layers. To survey potential DNA sequences involved in the activation of oleosin genes, a genomic clone of rice 18 kDa oleosin was sequenced, and its 5'-flanking region was compared with that of the known rice 16 kDa oleosin gene. Corresponding mRNAs of the two rice oleosin isoforms appeared seven days after pollination and vanished in mature seeds. Triacylglycerols and oleosins were accumulated concomitantly in maturing rice reeds in accord with the active assembly of oil bodies, and partly mobilized in postgerminative seedlings. Approximately 60% of the stored triacylglycerols in rice were not utilized: while the majority of oil bodies in embryos were mobilized in five days after imbibition, those in aleurone layers remained intact in postgerminative seedlings.

Citations

Jun 12, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Wen Xian LiuLe Qing Qu
Sep 2, 2005·Planta·Christina WaltersChristophe Bailly
Mar 17, 2011·Plant Molecular Biology·Mio KuwanoKaoru T Yoshida
Mar 2, 2010·Biological & Pharmaceutical Bulletin·Takashi L Shimada, Ikuko Hara-Nishimura
Sep 14, 2010·Bioscience, Biotechnology, and Biochemistry·Hsi-Chi LuJason T C Tzen
Mar 28, 2012·Bioscience, Biotechnology, and Biochemistry·Takanari ShigemitsuTakehiro Masumura
Feb 14, 2006·Transgenic Research·Junko Kohno-MuraseJun Imamura
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May 19, 2006·Plant & Cell Physiology·Arnaud LehnerChristophe Bailly
Oct 6, 2020·Frontiers in Plant Science·Miaomiao QinShenkui Liu

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