PMID: 11324750Apr 28, 2001Paper

Genomic comparison of an avirulent strain of Orf virus with that of a virulent wild type isolate reveals that the Orf virus G2L gene is non-essential for replication

Virus Genes
Colin J McInnesJ A Gilray

Abstract

The genomic structure of two strains of orf virus (OV), a field isolate (MRI-Scab) which has never been passaged in cell culture, and a multiple-passage cell culture-adapted strain (Orf-11) were compared. The Orf-11 genome is approximately 8.0 kb longer than that of the MRI-Scab due to a duplication of the right-hand end. The duplicated region has been translocated to the left-hand end of the genome with a loss of sequence from that end. The lost sequence contains three complete genes, namely E2L, E3L and G1L and 80% of a fourth gene, namely G2L. The sequence lost from G2L in Orf-11 has been replaced by a region of unrelated sequence, encoding 98 amino acids. Northern analysis shows that mRNA is expressed from this "new" gene. The two viruses were also compared for in vivo virulence and ability to protect against subsequent OV challenge. In vivo, the field isolate was fully virulent and conferred good protection against challenge, whereas the cell culture-adapted virus produced only mild lesions and reduced protection against challenge.

Citations

Mar 26, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M Büttner, H J Rziha
Oct 24, 2003·The Journal of General Virology·Ann R Wood, Colin J McInnes
Dec 13, 2003·Journal of Virology·G DelhonD L Rock
Aug 16, 2014·Veterinary Dermatology·Colin J McInnes
Aug 12, 2015·Virology Journal·Abdelmalik I KhalafallaYahia H Ali
Nov 12, 2015·Frontiers in Microbiology·Xuelin ChiShihua Wang
Oct 30, 2018·Reviews in Medical Virology·Ruixue WangShuhong Luo
Jan 9, 2019·BMC Veterinary Research·Guangxiang WangZhidong Zhang
Jan 29, 2010·The Journal of General Virology·Maria HautaniemiColin J McInnes
Dec 20, 2012·Expert Review of Vaccines·Veerakyathappa BhanuprakashRaj Kumar Singh
Jul 1, 2017·Journal of Virology·Artur YakimovichJason Mercer
Jul 22, 2020·The Journal of General Virology·Nicola F FletcherAlan W Baird
Aug 12, 2008·New Zealand Veterinary Journal·R A FairleyA A Mercer
Jan 25, 2003·Annual Review of Immunology·Bruce T SeetGrant McFadden
Sep 9, 2009·Expert Review of Anti-infective Therapy·Madhusudan HosamaniRaj Kumar Singh
Apr 19, 2002·The Journal of General Virology·R CottoneH-J Rziha
Jun 5, 2016·Comparative Immunology, Microbiology and Infectious Diseases·Thachamvally RiyeshRaj Kumar Singh
May 22, 2021·Animal Health Research Reviews·Zaharaddeen LawanMohd Lila Mohd-Azmi

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