Genomic comparison of Clostridium species with the potential of utilizing red algal biomass for biobutanol production

Biotechnology for Biofuels
Chongran SunYi-Rui Wu

Abstract

Sustainable biofuels, which are widely considered as an attractive alternative to fossil fuels, can be generated by utilizing various biomass from the environment. Marine biomass, such as red algal biomass, is regarded as one potential renewable substrate source for biofuels conversion due to its abundance of fermentable sugars (e.g., galactose). Previous studies focused on the enhancement of biofuels production from different Clostridium species; however, there has been limited investigation into their metabolic pathways, especially on the conversion of biofuels from galactose, via whole genomic comparison and evolutionary analysis. Two galactose-utilizing Clostridial strains were examined and identified as Clostridium acetobutylicum strain WA and C. beijerinckii strain WB. Via the genomic sequencing of both strains, the comparison of the whole genome together with the relevant protein prediction of 33 other Clostridium species was established to reveal a clear genome profile based upon various genomic features. Among them, five representative strains, including C. beijerinckii NCIMB14988, C. diolis DSM 15410, C. pasteurianum BC1, strain WA and WB, were further discussed to demonstrate the main differences among their respecti...Continue Reading

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Citations

Mar 19, 2019·Water Environment Research : a Research Publication of the Water Environment Federation·Youzhao WangTong Zhu
Sep 27, 2018·Biotechnology for Biofuels·Fengxue XinMin Jiang
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Mar 9, 2020·Journal of Molecular Microbiology and Biotechnology·Jin LiZhong Hu

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Methods Mentioned

BETA
phosphotransferase

Software Mentioned

Prodigal
P4
MetaPathways
MEGA6
Circos
GALACTUROCAT
Mauve
Adobe Illustrator
BLAST
SOAPdenovo

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