Genomic Comparison of Translocating and Non-Translocating Escherichia coli

PloS One
Nathan L BachmannAdam Polkinghorne

Abstract

Translocation of E. coli across the gut epithelium can result in fatal sepsis in post-surgical patients. In vitro and in vivo experiments have identified the existence of a novel pathotype of translocating E. coli (TEC) that employs an unknown mechanism for translocating across epithelial cells to the mesenteric lymph nodes and the blood stream in both humans and animal models. In this study the genomes of four TEC strains isolated from the mesenteric lymph nodes of a fatal case of hospitalised patient (HMLN-1), blood of pigs after experimental shock (PC-1) and after non-lethal haemorrhage in rats (KIC-1 and KIC-2) were sequenced in order to identify the genes associated with their adhesion and/or translocation. To facilitate the comparison, the genomes of a non-adhering, non-translocating E. coli (46-4) and adhering but non-translocating E. coli (73-89) were also sequenced and compared. Whole genome comparison revealed that three (HMLN-1, PC-1 and KIC-2) of the four TEC strains carried a genomic island that encodes a Type 6 Secretion System that may contribute to adhesion of the bacteria to gut epithelial cells. The human TEC strain HMLN-1 also carried the invasion ibeA gene, which was absent in the animal TEC strains and is l...Continue Reading

References

Mar 4, 2000·The Journal of Trauma·C G NettelbladtO Ljungqvist
Jun 1, 2000·Scandinavian Journal of Gastroenterology·M LjungdahlU Haglund
Dec 19, 2000·Infection and Immunity·B PicardF Taddei
Dec 20, 2000·Bioinformatics·K RutherfordB Barrell
Feb 14, 2004·Intensive Care Medicine·Simon FinferJohn Myburgh
Jun 25, 2005·Bioinformatics·Tim J CarverJulian Parkhill
May 13, 2006·Molecular Microbiology·Thierry WirthMark Achtman
Nov 29, 2007·The ISME Journal·Xiaoyan PangLiping Zhao
Oct 28, 2008·Cellular and Molecular Life Sciences : CMLS·T Proft, E N Baker
May 14, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M KatouliR Möllby
Jun 17, 2009·Cell Host & Microbe·Kimberly A KlineBirgitta Henriques-Normark
Feb 1, 2011·Bioinformatics·Mitchell J SullivanScott A Beatson
Jun 28, 2011·Applied and Environmental Microbiology·Margarita Martinez-MedinaChrista Ewers
Aug 10, 2011·BMC Genomics·Nabil-Fareed AlikhanScott A Beatson
Mar 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Eric Cascales, Christian Cambillau
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
Jun 26, 2012·Journal of Bacteriology·Maud FléchardPierre Germon
Feb 6, 2013·PloS One·Daniël J WurpelMark A Schembri

❮ Previous
Next ❯

Datasets Mentioned

BETA
MG1655
ATCC8739

Methods Mentioned

BETA
electrophoresis

Software Mentioned

Artemis Comparison Tool ( ACT )
tBLASTx
Spades
Easyfig
FASTQC
BLAST
MEGA5
Artemis
Muscle

Related Concepts

Related Papers

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
Mohammad KatouliR Möllby
Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition
Carl-Gustaf NettelbladtO Ljungqvist
© 2021 Meta ULC. All rights reserved