Genomic detection and characterization of a Korean isolate of Little cherry virus 1 sampled from a peach tree

Virus Genes
Seungmo LimJae Sun Moon

Abstract

A peach tree (Prunus persica) showing yellowing and mild mottle symptoms was analyzed using high-throughput RNA sequencing to determine the causal agent. A total of nine contigs similar to Little cherry virus 1 (LChV-1) were produced, and all the contigs showed nucleotide sequence identity (lower than 83 %) and query coverage (higher than 73 %) with LChV-1. The symptomatic peach sample was confirmed to be infected with LChV-1-like virus as a result of reverse transcription-polymerase chain reaction using primers designed based on sequences of the contigs. Occurrence of diseases caused by LChV-1 in Prunus species has been reported. Complete 16,931-nt genome of the peach virus composed of eight open reading frames was determined, and conserved domains including viral methyltransferase, viral helicase 1, RNA-dependent RNA polymerase (RdRp), heat shock protein 70 homologue (HSP70h), HSP90h and closterovirus coat protein (CP) were identified. Phylogenetic trees based on amino acid sequence alignments between the peach virus and members in the family Closteroviridae showed that the virus was most similar to LChV-1. Pairwise comparisons based on amino acid sequence alignments of three genes (RdRp, HSP70h and CP) between the peach viru...Continue Reading

References

Apr 20, 1995·Virology·A V KarasevR F Lee
Nov 10, 2001·Annual Review of Phytopathology·Alexander V Karasev
Dec 21, 2005·Virology·Theo W Dreher, W Allen Miller
Oct 24, 2008·Phytopathology·Ioannis E TzanetakisRobert R Martin
Dec 15, 2010·Virus Genes·Michael J MelzerJohn S Hu
Mar 8, 2013·Frontiers in Microbiology·Michael J MelzerJohn S Hu
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta

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