Genomic disruption of six budding yeast genes gives one drastic example of phenotype strain-dependence

Yeast
E BilslandP Sunnerhagen

Abstract

Using PCR to construct disruption cassettes, null alleles of six genes have been created in Saccharomyces cerevisiae. In a FY1679 background, no defects were detected in any of the haploid deletion mutants with respect to growth, gross morphology, or mating. A diploid FY1679-derived delta ygl194c/delta ygl194c homozygous disruptant displayed reduced sporulation. In contrast to the lack of phenotypic consequences of delta yol100w disruptions in the FY1679 background, in the CEN.PK2 strain even a heterozygous disruption of the same gene caused striking effects, very slow vegetative growth and highly impaired sporulation. Tetrad analysis showed YOL100w to be an essential gene in this strain. A copy of the YGL194c or the YOL100w wild-type gene borne on a centromeric episomal plasmid was introduced into a corresponding disruption mutant strain, and in both cases was found to partially complement the defects.

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
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Jan 1, 1991·Methods in Enzymology·F Sherman
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Citations

Jul 17, 2008·Comparative and Functional Genomics·A Rogowska-WrzesinskaS J Fey
Mar 2, 2011·BMC Biology·Michaela de ClareStephen G Oliver

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