Genomic DNA-chip hybridization reveals a higher incidence of genomic amplifications in pancreatic cancer than conventional comparative genomic hybridization and leads to the identification of novel candidate genes

Cancer Research
Karlheinz HolzmannMartin Bentz

Abstract

Genomic analyses aimed at the detection of high-level DNA amplifications were performed on 13 widely used pancreatic cancer cell lines and 6 pancreatic tumor specimens. For these analyses, array-based comparative genomic hybridization (Matrix-CGH) onto dedicated microarrays was used. In comparison with chromosomal CGH (eight amplifications), a >3-fold number of DNA amplifications was detected (n = 29). The most frequent amplifications mapped to 7p12.3 (three pancreatic cancer cell lines and three pancreatic tumor specimens), 8q24 (four pancreatic cancer cell lines and one pancreatic tumor specimen), 11q13 (three pancreatic cancer cell lines and three pancreatic tumor specimens), and 20q13 (four pancreatic cancer cell lines and three pancreatic tumor specimens). Genes contained in the consensus regions were MYC (8q24), EGFR (7p12.3), and FGF3 (11q13). In six of seven pancreatic cancer cell lines and pancreatic tumor specimens with 20q13 amplifications, the novel candidate gene NFAT C2, which plays a role in the activation of cytokines, was amplified. Other amplifications also affected genes for which a pathogenetic role in pancreatic carcinoma has not been described, such as BCL10 and BCL6, two members of the BCL family. A subse...Continue Reading

References

Jan 1, 1992·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·H P ElsässerH F Kern
Apr 1, 1992·Cancer·B JohanssonF Mitelman
Dec 1, 1987·Cancer Genetics and Cytogenetics·R CasaloneF Pasquali
Feb 1, 1994·Genes, Chromosomes & Cancer·C A GriffinC J Yeo
Jan 1, 1995·Cytometry·S du ManoirT Cremer
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·C CollinsJ W Gray
Aug 17, 1999·Seminars in Cancer Biology·M Schwab
Feb 15, 2001·International Journal of Cancer. Journal International Du Cancer·C WallrappT M Gress
May 31, 2001·Genome Research·C CollinsJ W Gray
Jan 18, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Swen WessendorfPeter Lichter
Jun 25, 2002·Nature Cell Biology·Sebastien JauliacAlex Toker
Oct 16, 2002·Genes, Chromosomes & Cancer·Eija H MahlamäkiAnne Kallioniemi
Nov 6, 2002·Japanese Journal of Cancer Research : Gann·Takafumi WatanabeJohji Inazawa
Dec 4, 2002·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Richard E LeachStephen A Krawetz
Jan 30, 2003·Oncogene·Olivier Albagli-Curiel
Jan 1, 2001·Methods in Molecular Medicine·M Baudis, M Bentz

❮ Previous
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Citations

Jul 7, 2005·Pathology Oncology Research : POR·László KopperJózsef Tímár
Nov 29, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Thomas M GressMalte Buchholz
Apr 7, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Zobeida Cruz-MonserrateKathleen L O'Connor
Dec 21, 2011·Nature Reviews. Gastroenterology & Hepatology·Nardin Samuel, Thomas J Hudson
Nov 17, 2005·European Journal of Human Genetics : EJHG·William W LockwoodWan L Lam
Dec 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Alec C KimmelmanRonald A DePinho
Oct 4, 2005·The Cancer Journal·Charles G MullighanJames R Downing
Sep 18, 2007·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Evi MichelsFrank Speleman
May 10, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·S A O'TooleJ J O'Leary
Dec 1, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jih-Hsiang LeeGiuseppe Giaccone
Aug 3, 2012·Nutrition & Metabolism·Ian Forrest Robey
Jun 26, 2007·PLoS Computational Biology·Oscar M Rueda, Ramón Díaz-Uriarte
Aug 22, 2007·PloS One·Ramón Díaz-Uriarte, Oscar M Rueda
Sep 3, 2011·PloS One·Kevin A KweiJonathan R Pollack
Nov 28, 2007·Journal of Clinical and Experimental Hematopathology : JCEH·Ming-Qing Du
Sep 18, 2008·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Cristóbal Belda-IniestaJaime Feliú
Apr 11, 2012·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·A M LuebkeN H Stoecklein
Jul 23, 2005·Neoplasia : an International Journal for Oncology Research·Murali D BashyamJonathan R Pollack
Jun 23, 2006·Neoplasia : an International Journal for Oncology Research·Myriam GoldsteinAvi Orr-Urtreger
Apr 11, 2009·Trends in Immunology·Hiromitsu Hara, Takashi Saito
Sep 27, 2008·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Malte BuchholzThomas M Gress
Feb 9, 2008·The Journal of Molecular Diagnostics : JMD·Georg Feldmann, Anirban Maitra
Jun 19, 2007·Brain & Development·Hye Sung KimHyoung Kyun Rha
Nov 4, 2015·Current Problems in Surgery·Shahid G Farid, Gareth Morris-Stiff
Aug 2, 2006·Molecular Microbiology·Hanjing YangJeffrey H Miller
Jul 21, 2012·International Journal of Cancer. Journal International Du Cancer·Jaroslaw SzczyrbaFriedrich A Grässer

❮ Previous
Next ❯

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