PMID: 8939029Nov 1, 1996Paper

Genomic DNA fingerprinting of clinical isolates of Helicobacter pylori using short oligonucleotide probes containing repetitive sequences

The Journal of Applied Bacteriology
D G MarshallC J Smyth

Abstract

The ability of oligonucleotide probes containing short repetitive sequence motifs to differentiate between isolates of Helicobacter pylori was investigated. Genomic DNA preparations from H. pylori were digested with the restriction enzyme HindIII, electrophoresed in agarose gels and transferred to nylon filters. Five separate oligonucleotide probes were tested for hybridization sequentially to fingerprint the digested DNA from a panel of 29 clinical isolates and one type strain of H. pylori, and their relative discriminatory abilities were assessed. Four probes, (GACA)4, (GT)8, (GTG)5 and (GGAT)4, were each shown to yield highly informative hybridization band profiles allowing differentiation of H. pylori isolates. The DNA fingerprints of individual isolates obtained with each probe were distinct and reproducible. Direct comparison with ribotyping revealed that oligonucleotide fingerprinting had far superior discriminatory power. Computer-assisted similarity analysis of (GGAT)4-generated hybridization profiles of pairwise combinations of H. pylori isolates revealed that there was no correlation between ribotype and oligonucleotide fingerprint patterns. The results of this study demonstrate that oligonucleotide probes containing...Continue Reading

References

Jun 1, 1992·Journal of Clinical Microbiology·W TeeB Dwyer
Jul 1, 1992·Postgraduate Medical Journal·H J O'Connor
Mar 1, 1990·Journal of Clinical Microbiology·J H OudbierC Bruin-Mosch
Jan 1, 1986·Digestive Diseases and Sciences·J W HamiltonP Bass
Sep 1, 1986·Journal of Clinical Microbiology·W LangenbergH C Zanen
Dec 1, 1984·Molecular and Cellular Biology·H HamadaC M Gorman
Feb 1, 1994·Journal of Clinical Microbiology·S FujimotoM J Blaser
May 1, 1994·Journal of Clinical Microbiology·I J WiidP D van Helden
May 25, 1994·Nucleic Acids Research·M R MicheliE D'Ambrosio
Dec 30, 1993·Biochemical and Biophysical Research Communications·G VenugopalS Mohapatra
Jul 1, 1993·Gastroenterology·D Y Graham, M F Go
Sep 1, 1995·Trends in Microbiology·E R Moxon

❮ Previous
Next ❯

Citations

May 26, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ian M CarrollNiyaz Ahmed
Jun 23, 1999·FEMS Immunology and Medical Microbiology·H ColdingK A Krogfelt
Feb 13, 2001·Infection and Immunity·D T PrideM J Blaser
Jul 11, 2006·Journal of Clinical Microbiology·Beata KrawczykJózef Kur
Aug 30, 2001·Journal of Clinical Microbiology·A M KlevytskaP Keim
Apr 12, 2003·Journal of Clinical Microbiology·Tomasz CieślikowskiWiesław Kaca
Jan 7, 2005·Annals of Clinical Microbiology and Antimicrobials·Niyaz Ahmed, Leonardo A Sechi
Mar 21, 2002·Expert Review of Molecular Diagnostics·L J van Doorn
Jan 10, 2009·World Journal of Gastroenterology : WJG·Sergio A ConReinaldo Con-Wong
Jul 29, 1999·Electrophoresis·M VosP D van Helden
Nov 5, 1999·Annual Review of Microbiology·Z Ge, D E Taylor
Aug 27, 2015·World Journal of Gastroenterology : WJG·Sahar Honarmand-JahromySaeid Latifi-Navid
May 17, 2005·Biochemistry. Biokhimii︠a︡·K T MomynalievV M Govorun
Jun 18, 2005·World Journal of Gastroenterology : WJG·Yue-Hua GongYuan Yuan
Mar 29, 2000·Alimentary Pharmacology & Therapeutics·B C WongS Lam

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.