PMID: 9441873Jan 27, 1998Paper

Genomic DNA from mice: a comparison of recovery methods and tissue sources

Biochemical and Molecular Medicine
J R HofstetterD K Lahiri

Abstract

Our aim is to identify an extraction method and the source of mouse tissue(s) that could allow a high-resolution genomic scan from a living mouse. We compared and optimized two methods for yield, purity of DNA, and their use in the polymerase chain reaction (PCR) of DNA extracted from different mouse tissues. In addition to whole blood, tissue samples from the brain, liver, testis, and tail were included in this study. The Rapid Method (RM) is preferable for the whole blood samples and testis and brain tissue samples because it is quicker, less toxic, and more cost-effective than the proteinase K method (PM). For liver the PM produced higher yields of DNA with less degradation than the RM. For tail tip, the PM produced a higher yield of DNA, but the RM resulted in a higher yield of PCR product. From a living mouse, a tail snip generated a sufficient amount of DNA for several hundred PCRs but not a complete genomic scan. We suggest that the RM can be used to extract genomic DNA for a complete genomic scan which requires either testicular tissues or repeated blood samples from the suborbital sinus over several months without sacrificing the animal.

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Citations

Jan 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T SakamotoA J Demetris
Dec 17, 2008·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Alan E Boyle, Kathryn J Gill
Sep 3, 2002·Journal of Biochemical and Biophysical Methods·Catherine A BurkhartMichelle Haber
Apr 7, 2000·Journal of Neuroscience Methods·J F LambertP J Quesenberry
Jan 29, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sergey S SereginAndrea Amalfitano
Jan 10, 2012·Nucleic Acids Research·Henrik LaurellMikael Kubista
Aug 15, 2009·Pharmacogenetics and Genomics·Heidi M B LesscherLouk J M J Vanderschuren
Dec 17, 2008·General and Comparative Endocrinology·Taro WatanabeYoshio Takei
Dec 13, 2006·The Journal of Investigative Dermatology·Sung June ByunTae-Yoon Kim
Aug 18, 2005·Analytical Biochemistry·Marlon R Schneider, Eckhard Wolf
Nov 18, 2005·Analytical Biochemistry·Chris MurgatroydDietmar Spengler
Mar 26, 2015·Primates; Journal of Primatology·Shuji Takabayashi, Hideki Katoh
Jun 11, 2005·BioTechniques·Steven J Clapcote, John C Roder
Feb 11, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eyal ZchariaIsrael Vlodavsky
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel M AppledornAndrea Amalfitano
Oct 15, 2020·MBio·Adele Goldman-PinkovichDan Zilberstein

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