Genomic DNA sequence, promoter expression, and chromosomal mapping of rat muscle carnitine palmitoyltransferase I

Genomics
D WangJ B McMillin

Abstract

Carnitine palmitoyltransferase I (CPT-I) is a key enzyme involved in the regulation of fatty acid oxidation. CPT-IA and CPT-IB are isoforms of carnitine palmitoyltransferase I, of which CPT-IA is expressed in liver, kidney, fibroblasts, and heart and CPT-IB is expressed in skeletal muscle, heart, brown and white adipocytes, and testes. Although the genomic DNA sequence of human CPT-IB is available, the transcription start site and upstream regulatory sequences are not known. For rat CPT-IB, only the cDNA sequence has been published. We have cloned the entire rat CPT-IB gene from a Lambda fix II rat kidney genomic library. The genomic structure contains 19 exons, with the transcription start site for CPT-IB located in a short first exon, which is a 13-bp extension to the previously published cDNA 5' sequence. The coding sequence is identical with the rat muscle cDNA. The rat CPT-IB gene contains 18 introns and 19 exons, the latter 18 exons showing 85% homology to the human CPT-IB cDNA. CPT-IB maps to rat chromosome 7 at band q34. A putative promoter region was identified to within 391 bp of the transcription start site. The muscle specificity of the 5' flanking region was verified by comparison of luciferase expression to that o...Continue Reading

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Citations

Nov 19, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Motoyuki IemitsuIwao Yamaguchi
Jul 17, 1999·The American Journal of the Medical Sciences·G A Cook, E A Park
Sep 26, 2000·Molecular Genetics and Metabolism·F R van der LeijB Bartelds
May 16, 2001·Genesis : the Journal of Genetics and Development·P A OverbeekA I Agoulnik
May 17, 2002·The Journal of Biological Chemistry·Feike R van der LeijNigel T Price
Jan 5, 2020·Endocrinology·Isabel R Schlaepfer, Molishree Joshi
Oct 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Motoyuki IemitsuIwao Yamaguchi
Mar 4, 2003·The Journal of Biological Chemistry·Meredith L MooreJeanie B McMillin
Aug 19, 2000·American Journal of Physiology. Endocrinology and Metabolism·M ZhouP F Pilch
Mar 21, 2001·Biochimica Et Biophysica Acta·R R RamsayF R van der Leij

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