Apr 18, 2020

Employing NaChBac for cryo-EM analysis of toxin action on voltage-gated Na+ channels in nanodisc

BioRxiv : the Preprint Server for Biology
S. GaoNieng Yan

Abstract

NaChBac, the first bacterial voltage-gated Na+ (Nav) channel to be characterized, has been the prokaryotic prototype for studying the structure-function relationship of Nav channels. Discovered nearly two decades ago, the structure of NaChBac has not been determined. Here we present the cryo-EM analysis of NaChBac in both detergent micelles and nanodiscs. Under both conditions, the conformation of NaChBac is nearly identical to that of the potentially inactivated NavAb. Determining the structure of NaChBac in nanodiscs enabled us to examine gating modifier toxins (GMTs) of Nav channels in lipid bilayers. To study GMTs in mammalian Navs, we generated a chimera in which the extracellular fragment of the S3 and S4 segments in the second voltage-sensing domain from Nav1.7 replaces the corresponding sequence in NaChBac. Cryo-EM structures of the nanodisc-embedded chimera alone and in complex with HuwenToxin IV (HWTX-IV) were determined to 3.5 [A] and 3.2 [A] resolutions, respectively. Compared to the structure of HWTX-IV-bound human Nav1.7, which was obtained at an overall resolution of 3.2 [A], the local resolution of the toxin has been improved from ~ 6 [A] to ~ 4 [A]. This resolution enabled visualization of toxin docking. NaChBa...Continue Reading

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Mentioned in this Paper

Genome
Genes
Helix (Snails)
Trematoda
Gene Expression
Genomics
Down-Regulation
Potamopyrgus antipodarum
Platyhelminthes
Snails

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