Genomic evidence that the live Chlamydia abortus vaccine strain 1B is not attenuated and has the potential to cause disease

Vaccine
D LongbottomH M B Seth-Smith

Abstract

The live, temperature-attenuated vaccine strain 1B of Chlamydia abortus, the aetiological agent of ovine enzootic abortion (OEA), has been implicated in cases of vaccine breakdown. The aim of this study was to understand the nature of this attenuation through sequencing of the vaccine parent strain (AB7) and the derived mutant strains 1B and 1H, as well as to clarify the role of the vaccine strain in causing disease through comparative whole genome analysis. Whole genome sequencing was performed on: vaccine parent strain AB7; N-methyl-N'-nitro-N-nitrosoguanidine (NTG)-induced temperature attenuated mutant strain 1B grown from the commercial live vaccines Cevac Chlamydia and Enzovax; strain 1H a reverted NTG mutant; and 5 strains isolated from cases of OEA originating from animals from the original vaccine safety trial (2 strains) or from vaccinated ewes or ewes exposed to vaccinated animals (3 strains). We confirmed that AB7 is in a different lineage from the reference strain S26/3. The genome of vaccine strain 1B contains ten single nucleotide polymorphisms (SNPs) created by the NTG treatment, which are identical to those found in strain 1H. The strains from OEA cases also cluster phylogenetically very tightly with these vacci...Continue Reading

Citations

Sep 17, 2020·Vector Borne and Zoonotic Diseases·Jian-Yong WuXue-Yun Yang
Jun 4, 2020·Veterinary Research·Sean R WattegederaGary Entrican
Feb 20, 2019·Frontiers in Microbiology·Sankhya Bommana, Adam Polkinghorne
Oct 27, 2020·Frontiers in Veterinary Science·Carlos MontbrauJesús Salinas

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Datasets Mentioned

BETA
LN589721
ERS200127
AB15
130861

Methods Mentioned

BETA
PCR
protein folding

Software Mentioned

GATK
MegAlign Pro Lasergene
Artemis Comparison Tool ( ACT )
DIVA
Artemis
Velvet

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