Genomic fingerprinting of Haemophilus somnus by a combination of PCR methods.

Journal of Clinical Microbiology
S AppuhamyH A Gibbs

Abstract

Twenty-three isolates of Haemophilus somnus were typed by repetitive extragenic palindromic (REP) element-based PCR, enterobacterial repetitive intergenic consensus (ERIC)-based PCR, and PCR ribotyping. A total of 11 types were distinguished by REP-PCR, 13 types were distinguished by ERIC-PCR, and 5 types were distinguished by PCR ribotyping. PCR ribotyping produced a relatively simple pattern and a small number of distinct types, whereas REP- and ERIC-PCR both produced more complex banding patterns but increased the discrimination between strains. Clearly distinguishable profiles were obtained for respiratory and genital isolates of H. somnus by all three typing methods. The results suggest that a combination of all three primer sets provides a high-resolution fingerprinting method for epidemiological studies of H. somnus and for its differentiation from related species.

References

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Jun 1, 1993·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·V Fussing, H C Wegener

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Citations

Feb 24, 2001·Australian Veterinary Journal·M RafieeP J Blackall
Sep 22, 2007·Foodborne Pathogens and Disease·Steven L FoleyRobert D Walker
Apr 5, 2005·The Journal of Veterinary Medical Science·Akinori TanakaYuichi Tagawa
Mar 7, 2003·Applied and Environmental Microbiology·Dhruva BhattacharyaBanwari Lal
Feb 6, 2018·Probiotics and Antimicrobial Proteins·Izildinha MorenoRenata Bromberg
Nov 18, 2017·International Journal of Environmental Research and Public Health·Jeeyeon LeeSoomin Lee
Mar 21, 1998·Journal of Clinical Microbiology·S AppuhamyR Parton
May 15, 1999·Journal of Clinical Microbiology·D M Olive, P Bean
Aug 17, 1999·Journal of Clinical Microbiology·S AlamD Beighton
Jul 11, 2006·Journal of Clinical Microbiology·Beata KrawczykJózef Kur

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