Genomic insights into the different layers of gene regulation in yeast.

Genetics Research International
José E Pérez-OrtínAntonio Jordán-Pla

Abstract

The model organism Saccharomyces cerevisiae has allowed the development of new functional genomics techniques devoted to the study of transcription in all its stages. With these techniques, it has been possible to find interesting new mechanisms to control gene expression that act at different levels and for different gene sets apart from the known cis-trans regulation in the transcription initiation step. Here we discuss a method developed in our laboratory, Genomic Run-On, and other new methods that have recently appeared with distinct technical features. A comparison between the datasets generated by them provides interesting genomic insights into the different layers of gene regulation in eukaryotes.

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Citations

Dec 21, 2011·Biochimica Et Biophysica Acta·José E Pérez-OrtínSebastián Chávez

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Methods Mentioned

BETA
dot
DNA array
immunoprecipitation
ChIP

Software Mentioned

Genomic Run - On ( GRO )
GRO
BioGRO
Nascent

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