Abstract
Putrescine is synthesized using one of two alternative pathways in plants, from arginine by arginine decarboxylase (ADC) or from ornithine by ornithine decarboxylase (ODC) and is catabolized by diamine oxidase (DAO). A survey of approximately 310,000 expressed sequenced tags (ESTs) in soybean EST libraries identified diverse representation of ADC, ODC, and DAO ESTs, with ODC being least frequent and DAO ESTs most abundant. Southern analysis suggested that ADC and ODC belong to small gene families, and DAO is the most divergent. Using three bacterial artificial chromosome (BAC) libraries, 26X genome equivalents, two common loci for ADC and DAO and one independent DAO locus were identified. ADC and DAO are physically linked in the soybean genome within approximately 150 kb. Identification of genomic regions encoding ODC proved difficult and required using additional BAC libraries, increasing genome coverage to approximately 40X. Using Real Time reverse transcriptase-polymerase chain reaction (RT-PCR), higher steady-state levels of ADC than ODC in roots, leaves, shoot apices, and dry seeds suggested that ADC is the predominant pathway for putrescine biosynthesis in soybean. However, organ-specific expression showed that root is th...Continue Reading
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