Genomic organization of the 5' end of human beta-ENaC and preliminary characterization of its promoter

American Journal of Physiology. Renal Physiology
Christie P ThomasOmar A Itani

Abstract

The mRNA for the beta-subunit of the epithelial Na(+) channel (beta-ENaC) is regulated developmentally and, in some tissues, in response to corticosteroids. To understand the mechanisms of transcriptional regulation of the human beta-ENaC gene, we characterized the 5' end of the gene and its 5'-flanking regions. Adaptor-ligated human kidney and lung cDNA were amplified by 5' rapid amplification of cDNA ends, and transcription start sites of two 5' variant transcripts were determined by nuclease protection or primer extension assays. Cosmid clones that contain the 5' end of the gene were isolated, and analysis of these clones indicated that alternate first exons approximately 1.5 kb apart and approximately 45 kb upstream of a common second exon formed the basis of these transcripts. Genomic fragments that included the proximal 5'-flanking region of either transcript were able to direct expression of a reporter gene in lung epithelia and to bind Sp1 in nuclear extracts, confirming the presence of separate promoters that regulate beta-ENaC expression.

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Citations

May 12, 2004·Molecular and Cellular Endocrinology·Fraser M RogersonPeter J Fuller
Aug 26, 2006·Biochemical and Biophysical Research Communications·Hisao YamamuraShoichi Shimada
Dec 16, 2003·American Journal of Respiratory Cell and Molecular Biology·Gail OtulakowskiHugh O'Brodovich
Apr 20, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·S J RammingerS M Wilson
Jun 23, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christie P ThomasRussell F Husted
Oct 1, 2011·European Journal of Pediatrics·Radovan BogdanovićMichael Ludwig
Sep 3, 2002·American Journal of Respiratory Cell and Molecular Biology·Christie P ThomasMichael Knowles
May 1, 2021·International Journal of Molecular Sciences·Silvia PierandreiMarco Lucarelli

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