Genomic organization, promoter activity of grass carp MDA5 and the association of its polymorphisms with susceptibility/resistance to grass carp reovirus

Molecular Immunology
Lan WangLimin Peng

Abstract

MDA5 (melanoma-differentiation-associated gene 5), a member of the RLR (retinoic acid-inducible gene I-like receptor) family, plays a pivotal role in innate immunity against viral infection. In the present study, the organization of grass carp (Ctenopharyngodon idella) MDA5 (CiMDA5) gene sequence was elucidated and promoter activity of its 5'-flanking region was detected. In addition, single nucleotide polymorphisms (SNPs) were investigated to explore their association with susceptibility/resistance to grass carp reovirus (GCRV). The CiMDA5 genomic sequence is composed of 10,906 bp, containing seven exons and six introns, which is distinct from its counterparts with numbers of exons. The 5'-flanking region of CiMDA5 gene, containing several putative transcription factor binding sites, was amplified using genome walking technique, exhibiting promoter activity. Six SNPs were discovered in the complete sequence of CiMDA5. Five of them locate in introns and one in 5'-flanking region. The genotype and allele distribution were examined by PCR-RFLP in susceptible and resistant fish. The results indicate that genotypes at the -713 C/G and 3338 A/C loci were significantly associated with the resistance to GCRV (P<0.05). Furthermore, at ...Continue Reading

References

Aug 5, 1998·Genetics·M S RöderM W Ganal
Dec 5, 2000·Genetics·L RamsayR Waugh
Mar 13, 2002·Nature Genetics·Franco PaganiFrancisco E Baralle
Apr 4, 2003·The Journal of Biological Chemistry·Sven HeinzMichael Rehli
Aug 14, 2003·Human Molecular Genetics·Bastiaan HoogendoornMichael C O'Donovan
Jun 1, 2004·Nature Reviews. Genetics·Franco Pagani, Francisco E Baralle
Jul 26, 2005·Immunity·Hiroki KatoShizuo Akira
Mar 11, 2006·Cell Death and Differentiation·C WertsD J Philpott
May 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Leonid GitlinMarco Colonna
Jul 11, 2006·Nature·Etienne MeylanMichael Karin
Dec 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Takeshi SaitoMichael Gale
Apr 12, 2007·Chest·Scott J TebbuttPeter D Paré
Apr 20, 2007·Arthritis Research & Therapy·Ioanna MarinouAnthony G Wilson
May 31, 2007·The Journal of Clinical Endocrinology and Metabolism·Alison SutherlandSimon H S Pearce
Oct 6, 2007·Immunologic Research·Capucine PicardJean-Laurent Casanova
Jan 31, 2008·Clinical Science·E Ann Misch, Thomas R Hawn
Feb 5, 2008·Molecular Cell·Sheng CuiKarl-Peter Hopfner
Feb 15, 2008·Current Opinion in Immunology·Osamu Takeuchi, Shizuo Akira
Feb 21, 2008·European Journal of Human Genetics : EJHG·Alfonso MartínezElena Urcelay
Oct 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Devanand SarkarPaul B Fisher
Jan 22, 2009·European Journal of Human Genetics : EJHG·Nicolas CouturierDavid Brassat
Apr 30, 2009·BMC Evolutionary Biology·Jun ZouChris J Secombes
Apr 28, 2010·Fish & Shellfish Immunology·Teng HuangHuiming Zhu
Dec 28, 2010·Developmental and Comparative Immunology·Maki OhtaniTakashi Aoki

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Citations

Mar 7, 2013·Fish & Shellfish Immunology·Takashi AokiTae Sung Jung
Aug 12, 2015·Fish & Shellfish Immunology·Tianle GuNana Yan
Apr 5, 2018·International Journal of Molecular Sciences·Hetron Mweemba Munang'anduLior David
Jun 3, 2021·Pathogens·Kizito K MugimbaHetron M Munang'andu

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