Genomic profile of urine has high diagnostic sensitivity compared to cytology in non-invasive urothelial bladder cancer

Cancer Science
Yosuke HirotsuMasao Omata

Abstract

Cytology is widely conducted for diagnosis of urothelial bladder cancer; however, its sensitivity is still low. Recent studies show that liquid biopsies can reflect tumor genomic profiles. We aim to investigate whether plasma or urine is more suitable for detecting tumor-derived DNA in patients with early-stage urothelial bladder cancer. Targeted sequencing of 71 genes was carried out using a total of 150 samples including primary tumor, urine supernatant, urine precipitation, plasma and buffy coat from 25 patients with bladder cancer and five patients with cystitis and benign tumor. We compared mutation profiles between each sample, identified tumor-identical mutations and compared tumor diagnostic sensitivities between urine and conventional cytology. We identified a total of 168 somatic mutations in primary tumor. In liquid biopsies, tumor-identical mutations were found at 53% (89/168) in urine supernatant, 48% (81/168) in urine precipitation and 2% (3/168) in plasma. The high variant allele fraction of urine was significantly related to worse clinical indicators such as tumor invasion and cytological examination. Although conventional cytology detected tumor cells in only 22% of non-invasive tumor, tumor diagnostic sensitiv...Continue Reading

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Citations

Sep 18, 2020·Current Opinion in Urology·Sharif HoseinQuoc-Dien Trinh
Dec 21, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Shuxing LvXuguo Sun
Dec 31, 2020·Cancer Communications·Hai-Ming Huang, Hai-Xia Li
Apr 4, 2021·Journal of Personalized Medicine·Matteo FerroDaniela Terracciano
Jun 6, 2021·Journal of Human Genetics·Kazunori WatanabeSiew-Kee Low

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Methods Mentioned

BETA
biopsy
biopsies
PCR
chip

Software Mentioned

R package

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