Genomic profiling of fungal cell wall-interfering compounds: identification of a common gene signature

BMC Genomics
Raúl GarcíaJ Arroyo

Abstract

The fungal cell wall forms a compact network whose integrity is essential for cell morphology and viability. Thus, fungal cells have evolved mechanisms to elicit adequate adaptive responses when cell wall integrity (CWI) is compromised. Functional genomic approaches provide a unique opportunity to globally characterize these adaptive mechanisms. To provide a global perspective on these CWI regulatory mechanisms, we developed chemical-genomic profiling of haploid mutant budding yeast cells to systematically identify in parallel those genes required to cope with stresses interfering the cell wall by different modes of action: β-1,3 glucanase and chitinase activities (zymolyase), inhibition of β-1,3 glucan synthase (caspofungin) and binding to chitin (Congo red). Measurement of the relative fitness of the whole collection of 4786 haploid budding yeast knock-out mutants identified 222 mutants hypersensitive to caspofungin, 154 mutants hypersensitive to zymolyase, and 446 mutants hypersensitive to Congo red. Functional profiling uncovered both common and specific requirements to cope with different cell wall damages. We identified a cluster of 43 genes highly important for the integrity of the cell wall as the common "signature of c...Continue Reading

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Citations

Feb 10, 2017·Archives of Toxicology·C C ChangS C-A Chen
Jan 24, 2018·Antimicrobial Agents and Chemotherapy·Sumanun SuwunnakornElena Rustchenko
Mar 28, 2018·Scientific Reports·Fabiana BrandãoJ Andrew Alspaugh
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Jan 21, 2016·The Journal of Biological Chemistry·Esmeralda Alonso-RodríguezHumberto Martín
Oct 11, 2019·Antonie van Leeuwenhoek·Maíse Gomes QueirozMarcos Antonio de Morais
Jul 12, 2017·Mediators of Inflammation·Juliana Alves Parente-RochaMaristela Pereira
Feb 2, 2020·Food Chemistry·Shinsuke NeromeMasayuki Azuma
May 5, 2021·Antonie van Leeuwenhoek·Maise Gomes QueirozMarcos Antonio de Morais Junior
Jul 5, 2021·Microbial Cell Factories·Vanessa S D CarvalhoJuan Carlos G Cortés
Oct 2, 2021·Applied Microbiology and Biotechnology·Lili ShuHui Zeng

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Methods Mentioned

BETA
GTPase
histone acetylation
ubiquitination
biosensors

Software Mentioned

GO Term Finder
GraphPad
Gene Ontology term finder
CAS
Multiexperiment Viewer ( MeV
GO Slim Mapper

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