Genomic relatedness of Staphylococcus pettenkoferi isolates of different origins

Journal of Medical Microbiology
Emeli MånssonBo Söderquist

Abstract

The aim of the study was to characterize clinical and environmental Staphylococcus pettenkoferi isolates with regard to genomic diversity and antibiotic susceptibility pattern. Repetitive-sequence-based PCR and core genome phylogenetic analysis of whole-genome sequencing (WGS) data verified the presence of distinct clades comprising closely related S. pettenkoferi isolates from different geographical locations and origins. Phylogenetic relationships between 25 S. pettenkoferi isolates collected from blood cultures and intra-operative air sampling were determined by repetitive-sequence-based PCR typing and analysis of ~157 000 SNPs identified in the core genome after WGS. Antibiotic susceptibility testing and tests for biofilm production (microtitre plate assay) were performed. Repetitive-sequence-based PCR as well as WGS data demonstrated the close relatedness of clinically significant blood culture isolates to probable contaminants, as well as to environmental isolates. Antibiotic-susceptibility testing demonstrated a low level of antimicrobial resistance. The mecA gene was present in two cefoxitin-resistant isolates. No isolates were found to produce biofilm. Close genomic relatedness of S. pettenkoferi isolates from differen...Continue Reading

References

Jul 11, 2002·Diagnostic Microbiology and Infectious Disease·Konrad TrülzschJürgen Heesemann
May 11, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C BerglundB Söderquist
Jan 5, 2007·Journal of Clinical Microbiology·Caroline LoïezRené J Courcol
Jul 13, 2007·International Journal of Systematic and Evolutionary Microbiology·Konrad TrülzschKarsten Becker
Jan 15, 2009·Journal of Medical Microbiology·Sang Hoon SongEui Chong Kim
Mar 17, 2011·Nature Reviews. Microbiology·Elizabeth A Grice, Julia A Segre
Apr 18, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P Nordmann, G Cornaglia
Oct 17, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D RaoultG Kahlmeter
Jan 26, 2013·PloS One·Hermine V MkrtchyanRonald R Cutler
Oct 18, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E MatuschekG Kahlmeter
Jan 1, 2011·Case Reports in Critical Care·Caterina MamminaDaniela Maria Palma
Oct 4, 2014·Clinical Microbiology Reviews·Karsten BeckerGeorg Peters
May 9, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Emeli MånssonBo Söderquist

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Citations

Dec 12, 2018·Veterinary World·Tapan Kumar DuttaSuvendu Kumar Behera
Jan 24, 2021·Diagnostic Microbiology and Infectious Disease·Uzoamaka A EkeHossein Salimnia
Oct 12, 2021·Frontiers in Microbiology·Alicja WysockaIzabela Sabała

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Methods Mentioned

BETA
total hip arthroplasty
PCR
PCRs
electrophoresis

Software Mentioned

SENTRY
CLC Genomics Workbench
FastTree
PhyML
MUMmer
DiversiLab

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