PMID: 9524816Jan 1, 1997Paper

Genomic sequence and mapping of a methyljasmonate-induced O-methyltransferase from barley (Hordeum vulgare L.)

DNA Sequence : the Journal of DNA Sequencing and Mapping
J E LeeM Löbler

Abstract

We have isolated a genomic clone corresponding to a caffeic acid O-methyltransferase (COMT) from barley (Hordeum vulgare L.) using a cDNA for a previously described jasmonate-regulated mRNA showing homology to COMT. Primer extension was used to characterize the 5' end of the mRNA while the 3' end, intron/exon structure and other features of the sequence were deduced by comparison to the cDNA sequence and/or conserved motifs. The gene is mapped to chromosome five and is absent in the barley cultivar Morex. Southern and northern analyses suggest that no differences in genomic structure and jasmonate inducibility exist between the barley cultivar Salome (source of the cDNA clone) and Igri (source of the genomic clone). This genomic clone is thus suitable for promoter studies with respect to jasmonate induction.

References

Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H GundlachM H Zenk
Jul 1, 1995·The Plant Cell·R. Whetten, R. Sederoff
Jul 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·A KleinhofsB J Steffenson

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Citations

Aug 26, 2009·Plant Signaling & Behavior·Atsushi IshiharaKyo Wakasa

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