Genomic Sequencing of Bordetella pertussis for Epidemiology and Global Surveillance of Whooping Cough

Emerging Infectious Diseases
Valérie BouchezSylvain Brisse

Abstract

Bordetella pertussis causes whooping cough, a highly contagious respiratory disease that is reemerging in many world regions. The spread of antigen-deficient strains may threaten acellular vaccine efficacy. Dynamics of strain transmission are poorly defined because of shortcomings in current strain genotyping methods. Our objective was to develop a whole-genome genotyping strategy with sufficient resolution for local epidemiologic questions and sufficient reproducibility to enable international comparisons of clinical isolates. We defined a core genome multilocus sequence typing scheme comprising 2,038 loci and demonstrated its congruence with whole-genome single-nucleotide polymorphism variation. Most cases of intrafamilial groups of isolates or of multiple isolates recovered from the same patient were distinguished from temporally and geographically cocirculating isolates. However, epidemiologically unrelated isolates were sometimes nearly undistinguishable. We set up a publicly accessible core genome multilocus sequence typing database to enable global comparisons of B. pertussis isolates, opening the way for internationally coordinated surveillance.

References

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Citations

Jul 23, 2019·Journal of Medical Microbiology·Ikram Ben FrajSylvain Brisse
Apr 20, 2019·Microbiology Resource Announcements·Shweta AlaiSunil Gairola
May 10, 2020·BMC Genomics·Shweta AlaiSunil Gairola
Feb 25, 2021·Emerging Infectious Diseases·Adriana CabalWerner Ruppitsch
Feb 26, 2021·Journal of Clinical Microbiology·Michael R WeigandM Lucia Tondella
Sep 18, 2021·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Valérie BouchezSylvain Brisse

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Methods Mentioned

BETA
genotyping
electrophoresis

Software Mentioned

cgMLST
PFGE
aLRT
MLVA
BioNumerics

Related Concepts