Genomic structural variations contribute to trait improvement during whole-genome shuffling of yeast

Applied Microbiology and Biotechnology
Dao-Qiong ZhengXue-Chang Wu

Abstract

Whole-genome shuffling (WGS) is a powerful technology of improving the complex traits of many microorganisms. However, the molecular mechanisms underlying the altered phenotypes in isolates were less clarified. Isolates with significantly enhanced stress tolerance and ethanol titer under very-high-gravity conditions were obtained after WGS of the bioethanol Saccharomyces cerevisiae strain ZTW1. Karyotype analysis and RT-qPCR showed that chromosomal rearrangement occurred frequently in genome shuffling. Thus, the phenotypic effects of genomic structural variations were determined in this study. RNA-Seq and physiological analyses revealed the diverse transcription pattern and physiological status of the isolate S3-110 and ZTW1. Our observations suggest that the improved stress tolerance of S3-110 can be largely attributed to the copy number variations in large DNA regions, which would adjust the ploidy of yeast cells and expression levels of certain genes involved in stress response. Overall, this work not only constructed shuffled S. cerevisiae strains that have potential industrial applications but also provided novel insights into the molecular mechanisms of WGS and enhanced our knowledge on this useful breeding strategy.

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Citations

Mar 7, 2014·Applied Microbiology and Biotechnology·Damien Biot-Pelletier, Vincent J J Martin
Dec 6, 2014·Journal of Industrial Microbiology & Biotechnology·Ke ZhangDaoqiong Zheng
Jan 13, 2015·Current Opinion in Biotechnology·Andreas K Gombert, Antonius J A van Maris
Jun 10, 2015·Current Opinion in Chemical Biology·Jie ZhangJay D Keasling
Apr 15, 2014·FEMS Microbiology Reviews·Jan SteenselsKevin J Verstrepen
Jan 31, 2015·Applied Microbiology and Biotechnology·Wenshan Liu, Rongrong Jiang
Mar 28, 2017·Applied and Environmental Microbiology·Arthur R Gorter de VriesJean-Marc G Daran
Jan 16, 2018·Applied Microbiology and Biotechnology·Ke ZhangDao-Qiong Zheng
Sep 26, 2019·FEMS Yeast Research·Arthur R Gorter de VriesJean-Marc G Daran
Aug 21, 2018·Biotechnology for Biofuels·Carlos H Luna-FloresEsteban Marcellin
Apr 22, 2017·Applied Microbiology and Biotechnology·Ke ZhangXue-Chang Wu
Jun 26, 2020·Frontiers in Genetics·Arthur R Gorter de VriesJean-Marc G Daran
Oct 26, 2018·Biotechnology for Biofuels·Damien Biot-PelletierVincent J J Martin
Jan 21, 2021·International Journal of Molecular Sciences·Xing-Xing TangDao-Qiong Zheng

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