Genomic structure and chromosomal localization of the novel ETS factor, PE-2 (ERF)

Genomics
C M de CastroR E Kaufman

Abstract

The members of the ETS family of transcription factors are grouped because they share a highly conserved DNA binding domain. These factors are involved in growth factor pathways and regulate both proliferation and differentiation. To identify ETS factors that may be involved in early hematopoietic progenitor regulation, we isolated a novel member of the ETS family by reverse transcriptase-PCR of the conserved DNA binding domain using degenerate oligonucleotides. This gene directs the synthesis of a 2704-nucleotide transcript whose largest open reading frame encodes a 548-amino-acid protein. Northern blot analysis reveals ubiquitous expression in all human tissues and cell lines tested, with highest levels in the testis, ovary, pancreas, and heart. Comparison of this gene with the available databases reveals very significant homology to the ETS factor PE-1 and probable near-identity with the recently cloned factor ERF. The PE-2 gene is composed of four exons spanning over 9 kb of genomic DNA. Sequence analysis of the promoter region reveals a GC-rich sequence without a TATA motif and with putative binding motifs for CREB, c-myb, and AP-1 factors. Using mouse-human somatic hybrids and FISH analysis, the PE-2 gene is localized to ...Continue Reading

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Oct 23, 2004·Nucleic Acids Research·Peter C HollenhorstBarbara J Graves
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Jun 28, 2020·American Journal of Medical Genetics. Part a·Aude-Annick SuterNadja Ehmke
Nov 12, 2020·Biochemistry·Caixia HouOleg V Tsodikov

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