Genomic structure and expression analyses of serine acetyltransferase gene in Citrullus vulgaris (watermelon)

Gene
K SaitoM Noji

Abstract

The genomic clones of Sat gene encoding serine acetyltransferase (SATase), a key enzyme in cysteine biosynthesis in plants, were isolated from the genomic library of Citrullus vulgaris (watermelon). The determination of nucleotide sequence of 5.7 kilobase pair (kbp) length revealed the presence of two introns of 1939 basepair (bp) and 515 bp length in the gene. The transcription start point was determined by primer extension experiments. Southern blot analysis indicated the presence of a single copy of the Sat gene and a couple of additional related sequences in the genome of C. vulgaris. The expression of Sat was analyzed in watermelon plants growth under sulfur- and/or nitrogen-starved conditions and in the presence of pyrazole, O-acetylserine and N-acetylserine. Only slight increment (ca. 1.5-2-fold) of Sat gene expression was observed upon sulfur starvation for 48 h. Interestingly, the addition of pyrazole, which is a precursor of beta-pyrazolealanine (beta-PA) synthesized by SATase and cysteine/beta-PA synthase, enhanced the expression of Sat by ca. 2-fold.

References

Feb 9, 1978·Nature·W Gilbert
Jun 1, 1989·The Plant Cell·R D AllenR N Beachy
Jul 23, 1982·Science·S L McKnight, R Kingsbury

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Citations

Nov 25, 2005·Photosynthesis Research·Markus Wirtz, Michel Droux
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·H TakahashiK Saito
Nov 4, 2006·Plant, Cell & Environment·Malcolm J Hawkesford, Luit J De Kok
Jan 10, 2018·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kazuki Saito
Sep 6, 2011·Prehospital and Disaster Medicine·Amy H SchnallSusan T Cookson

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