Abstract
Human type-2 tissue factor pathway inhibitor (TFPI-2), also known as placental protein 5, is a 32 kDa serine proteinase inhibitor consisting of three tandemly arranged Kunitz-type inhibitor domains homologous to tissue factor pathway inhibitor. TFPI-2 strongly inhibits a wide variety of serine proteinases including trypsin, chymotrypsin, plasmin, kallikrein and blood coagulation factor XIa. In this study, we have isolated and characterized a genomic clone from an artificial chromosome genomic library that encodes the entire human TFPI-2 gene. The human TFPI-2 gene spans approximately 7 kb and consists of five exons and four introns. Each Kunitz-type domain is encoded by a single exon, similar to that observed for murine TFPI-2 and other Kunitz-type proteinase inhibitors. A total of 535 bp of the 3'-flanking region contain two probable polyadenylation sites (AATAAA) at +4297 and +4314. A single transcription initiation site was identified by oligo-capping and reverse transcription-PCR analysis. Transient transfection of reporter plasmids containing segments of the 5'-flanking region into human transformed bone marrow endothelial cells and glioblastoma cells identified an 85 bp region (-224 to -139) sufficient for transcription o...Continue Reading
References
Sep 1, 1992·Experimental Cell Research·S VukicevicA H Reddi
Jun 1, 1989·Journal of Cellular Biochemistry·P G McGuire, N W Seeds
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·I VlodavskyM Klagsbrun
Jan 22, 1982·Nucleic Acids Research·S M Mount
May 10, 1995·Archives of Biochemistry and Biophysics·C N RaoU P Thorgeirsson
Mar 1, 1995·The Journal of Investigative Dermatology·C N RaoD T Woodley
Nov 1, 1994·Journal of Biochemistry·Y MiyagiK Miyazaki
Aug 1, 1993·Genomics·K EnjyojiH Kato
Jan 28, 1994·Gene·K Maruyama, S Sugano
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C A SprecherD C Foster
Mar 1, 1993·Journal of Cellular Physiology·G KornerI Vlodavsky
Jan 9, 1996·Biochemistry·L C PetersenW Kisiel
Jul 1, 1996·Genomics·Y MiyagiK Miyazaki
Nov 1, 1996·Archives of Biochemistry and Biophysics·C N RaoD T Woodley
Nov 1, 1996·DNA and Cell Biology·Y MiyagiK Miyazaki
Jan 28, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·M IinoW Kisiel
Apr 21, 1998·Placenta·K UdagawaK Miyazaki
Jul 27, 1999·The Journal of Biological Chemistry·H Nagase, J F Woessner
Citations
Oct 26, 2005·Annals of Hematology·Sabine SieglingChristian Götting
Mar 21, 2006·BMC Genomics·Kristiina A M VuoriMikko Nikinmaa
Dec 7, 2007·BMC Molecular Biology·Hongshen GuoDuan Ma
Nov 27, 2014·PeerJ·Salvatore Andrea MastroliaOffer Erez
Dec 17, 2008·Atherosclerosis·Christophe ZawadzkiBrigitte Jude
Jun 9, 2004·Thrombosis Research·Sabine SieglingKnut Kleesiek
Mar 21, 2007·International Journal of Cancer. Journal International Du Cancer·Yoshimasa NobeyamaToshikazu Ushijima
Jun 26, 2013·Developmental Biology·Yanli ZhangDuan Ma
May 25, 2013·Nucleic Acids Research·Hazel A CruickshanksCristina Tufarelli
Nov 4, 2009·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Offer ErezSonia S Hassan
Mar 1, 2005·Oncogene·Federico A SteinerDavid S Schrump
Jan 19, 2013·Blood·Satish KhuranaCatherine M Verfaillie
Feb 13, 2002·Oncogene·Santhi D KonduriJasti S Rao
Oct 27, 2015·Medicine·Jia YuHuan-Chun Ni
Nov 26, 2002·The Journal of Biological Chemistry·Christina KastMalcolm Whiteway
Nov 18, 2008·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Offer ErezRoberto Romero
Dec 10, 2008·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Offer ErezJuan Pedro Kusanovic
Jan 9, 2014·Cancer Research·Abdessamad ZerrouqiErwin G Van Meir
May 22, 2003·Thrombosis Research·F HubéY Gruel