Genomic structure and promoter activity of the mouse polysialic acid synthase gene (mST8Sia II). Brain-specific expression from a TATA-less GC-rich sequence.

The Journal of Biological Chemistry
Y YoshidaS Tsuji

Abstract

The mouse ST8Sia II (mST8Sia II/STX) gene encodes a neural cell adhesion molecule-specific polysialic acid synthase whose expression is regulated during the developmental stages of mouse brain. To elucidate the molecular mechanism by which the expression is tissue-specifically and developmentally regulated, we isolated the complete genomic DNA and characterized the promoter of the gene for mST8Sia II. The gene encoding mST8Sia II was found to span about 80 kilobases and to be composed of six exons. Primer extension and S1 nuclease protection analyses revealed that the transcription started from 167 nucleotides upstream of the translational initiation site. Promoter analyses of the 5'-flanking region of the mST8Sia II gene using a luciferase gene reporter system revealed strong promoter activity in retinoic acid-induced differentiated P19 cells, which highly express the mST8Sia II gene. Deletion analyses demonstrated that the minimal promoter activity detected for the proximal region 325 base pairs upstream from the translational initiation codon (-158 to +167) could be modulated by various sequences within the 9. 5-kilobase 5'-flanking region. The minimal promoter was embedded in a GC-rich domain (74%, GC content), in which two...Continue Reading

References

Feb 1, 1992·Glycobiology·F A Troy
Apr 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·M T MinowaM M Mouradian
Apr 1, 1991·Molecular and Cellular Biology·J D SafferM B Annarella
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·I A MastrangeloP V Hough
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Apr 15, 1989·European Journal of Biochemistry·M VibertH Skala
Aug 1, 1988·Molecular and Cellular Biology·A SehgalM Chao
Sep 29, 1995·The Journal of Biological Chemistry·E P ScheideggerJ B Lowe
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·J NakayamaM Fukuda
Jun 16, 1995·The Journal of Biological Chemistry·Y YoshidaS Tsuji
Jan 27, 1995·The Journal of Biological Chemistry·A K Datta, J C Paulson
Feb 23, 1995·Nature·M EckhardtR Gerardy-Schahn
Jan 26, 1996·The Journal of Biological Chemistry·J Nakayama, M Fukuda
Mar 22, 1996·The Journal of Biological Chemistry·H KitagawaK Sugahara
Oct 1, 1996·Glycobiology·S TsujiJ C Paulson

❮ Previous
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Citations

May 20, 1998·Molecular Neurobiology·K C BreenF D Hayes
Apr 8, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J G GeislerM L Mucenski
Sep 22, 2009·Glycoconjugate Journal·Sylvain LehouxMarie-Ange Krzewinski-Recchi
Jun 3, 1999·Brain Research. Molecular Brain Research·Z FengJ S Hong
Feb 19, 1998·Biochimica Et Biophysica Acta·T M MaguireK C Breen
Feb 7, 1998·Current Opinion in Neurobiology·J Z Kiss, G Rougon
Nov 18, 1998·Current Opinion in Structural Biology·M MühlenhoffR Gerardy-Schahn
Sep 22, 1999·European Journal of Biochemistry·N KurosawaT Muramatsu
Dec 22, 1998·Glycobiology·M Eckhardt, R Gerardy-Schahn
Feb 14, 1998·European Journal of Biochemistry·T HirokiK Tsukada
May 8, 2008·Bioscience, Biotechnology, and Biochemistry·Shou Takashima
May 8, 2008·Neurochemical Research·Herbert HildebrandtRita Gerardy-Schahn
Apr 24, 2016·Biochimica Et Biophysica Acta·Chihiro SatoKen Kitajima
Mar 13, 1999·The Journal of Biological Chemistry·H JiangM C Lucy
May 23, 1998·The Journal of Biological Chemistry·A K DattaJ C Paulson
Apr 20, 2000·The Journal of Biological Chemistry·C S MayanilE G Bremer
Nov 8, 2001·DNA Sequence : the Journal of DNA Sequencing and Mapping·S BernauerE Falkenstein
Mar 10, 1998·The Journal of Comparative Neurology·D W Allan, J J Greer
Jul 23, 2019·FEBS Letters·Prathyush PothukuchiSeetharaman Parashuraman
Oct 9, 2001·The Journal of Biological Chemistry·C S MayanilE G Bremer
Apr 29, 1998·The Journal of Biological Chemistry·S TakashimaS Tsuji
May 8, 2001·The Journal of Biological Chemistry·K Borges, R Dingledine
May 1, 2002·The Journal of Biological Chemistry·Shou TakashimaMasafumi Tsujimoto
Jun 22, 2006·The Journal of Biological Chemistry·Chandra S K MayanilEric G Bremer

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