PMID: 11320062Apr 26, 2001Paper

Genomic structure and transcriptional regulation of human Galbeta1,3GalNAc alpha2,3-sialyltransferase (hST3Gal I) gene

Glycobiology
A TaniguchiK Matsumoto

Abstract

Previous studies have shown that hST3Gal I mRNA is overexpressed in colorectal cancer tissues and primary breast carcinoma compared with nonmalignant or benign tissue, suggesting that the transcriptional regulation of hST3Gal I gene is altered during malignant transformation. We report transcriptional regulation of the hST3Gal I gene in colon adenocarcinoma and leukemia cell lines. To determine the genomic structure of the 5'-untranslated region, we cloned and identified the 5'-untranslated region of hST3Gal I from a human genome library. The 5'-untranslated region was found to be divided into three exons, namely, exons Y, X, and C1. The transcription initiation sites map at -1035 bp from the translation initiation site. Our results indicate that the transcriptional regulation of hST3Gal I depends on the pI promoter that exists 5'-upstream of exon Y in these cell lines. The results of luciferase assay suggest that the nt -304 to -145 region is important for transcriptional activity of hST3Gal I gene in both cell lines. The nt -304 to -145 region contains two sequences similar to the Sp1 recognition elements (GC-box) and one USF binding site. The results of site-directed mutagenesis indicated that the Sp1 binding sites and USF b...Continue Reading

References

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Citations

Dec 31, 2002·Biochemical and Biophysical Research Communications·Akiyoshi TaniguchiKojiro Matsumoto
Jul 25, 2003·Biochemical and Biophysical Research Communications·Liming XuAkiyoshi Taniguchi
Jan 1, 2013·The Journal of Biological Chemistry·Daisy SprovieroJoy M Burchell
May 31, 2014·World Journal of Gastroenterology : WJG·Yoshinori InagakiWei Tang
May 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tetsufumi KoikeReiji Kanangi
Nov 18, 2014·Cell Structure and Function·Mai TaniguchiHiderou Yoshida
Jan 29, 2009·International Journal of Cancer. Journal International Du Cancer·Yoshifumi KawaguchiYasufumi Kaneda
Oct 4, 2005·Biochimica Et Biophysica Acta·Anna DabrowskaMaciej Ugorski
Feb 5, 2003·Biochemical and Biophysical Research Communications·Akiyoshi TaniguchiKojiro Matsumoto
Aug 16, 2019·Molecular Omics·Shikha AcharyaNiclas G Karlsson

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