Genomic structure, promoter identification, and chromosomal mapping of a mouse nuclear orphan receptor expressed in embryos and adult testes

Genomics
C H LeeL N Wei

Abstract

We have isolated and characterized overlapping genomic clones containing the complete transcribed region of a newly isolated mouse cDNA encoding an orphan receptor expressed specifically in midgestation embryos and adult testis. This gene spans a distance of more than 50 kb and is organized into 13 exons. The transcription initiation site is located at the 158th nucleotide upstream from the translation initiation codon. All the exon/intron junction sequences follow the GT/AG rule. Based upon Northern blot analysis and the size of the transcribed region of the gene, its transcript was determined to be approximately 2.5 kb. Within approximately 500 bp upstream from the transcription initiation site, several immune response regulatory elements were identified but no TATA box was located. This gene was mapped to the distal region of mouse chromosome 10 and its locus has been designated Tr2-11. Immunohistochemical studies show that the Tr2-11 protein is present mainly in advanced germ cell populations of mature testes and that Tr2-11 gene expression is dramatically decreased in vitamin A-depleted animals.

Citations

Aug 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Pawan GuptaLi-Na Wei
Jun 8, 2002·Molecular and Cellular Biology·Chih-Rong ShyrChawnshang Chang
Dec 26, 2006·Nature Structural & Molecular Biology·Sung Wook ParkLi-Na Wei
Jul 3, 2002·The Journal of Biological Chemistry·Yueh-Chiang HuChawnshang Chang
Jun 26, 2008·American Journal of Physiology. Endocrinology and Metabolism·Ian A SimpsonSusan J Vannucci
Jun 26, 2008·American Journal of Physiology. Endocrinology and Metabolism·Zhiming HanKeith E Latham
Jul 11, 1998·The Journal of Biological Chemistry·C ChinpaisalL N Wei
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·A R Leitch
Apr 15, 2000·The Journal of Biological Chemistry·L N WeiC Chinpaisal
Feb 5, 1999·Progress in Retinal and Eye Research·J Graw
Oct 4, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Yi-Fen LeeChawnshang Chang

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