Genomic variation in the porcine immunoglobulin lambda variable region

Immunogenetics
Xi GuoMichael P Murtaugh

Abstract

Production of a vast antibody repertoire is essential for the protection against pathogens. Variable region germline complexity contributes to repertoire diversity and is a standard feature of mammalian immunoglobulin loci, but functional V region genes are limited in swine. For example, the porcine lambda light chain locus is composed of 23 variable (V) genes and 4 joining (J) genes, but only 10 or 11 V and 2 J genes are functional. Allelic variation in V and J may increase overall diversity within a population, yet lead to repertoire holes in individuals lacking key alleles. Previous studies focused on heavy chain genetic variation, thus light chain allelic diversity is not known. We characterized allelic variation of the porcine immunoglobulin lambda variable (IGLV) region genes. All intact IGLV genes in 81 pigs were amplified, sequenced, and analyzed to determine their allelic variation and functionality. We observed mutational variation across the entire length of the IGLV genes, in both framework and complementarity determining regions (CDRs). Three recombination hotspot motifs were also identified suggesting that non-allelic homologous recombination is an evolutionarily alternative mechanism for generating germline antib...Continue Reading

References

Oct 6, 1978·Science·J G SeidmanP Leder
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1989·Annual Review of Genetics·T K Blackwell, F W Alt
Nov 20, 1987·Science·F W AltG D Yancopoulos
Aug 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M YerleJ Gellin
Apr 25, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·T P KennyD L Robbins
Aug 12, 2003·Molecular Immunology·Mats Ohlin, Moncef Zouali
Jan 6, 2005·Springer Seminars in Immunopathology·Nicole BaumgarthLeonore A Herzenberg
Nov 16, 2005·Annual Review of Genetics·Nancy Maizels
Jun 19, 2009·Molecular Biology and Evolution·O RamírezM Amills
Mar 30, 2010·Veterinary Immunology and Immunopathology·Patricia BoydJoan K Lunney
May 1, 2009·Molecular Ecology Resources·Wiesław BabikJacek Radwan
Sep 10, 2011·Bioinformatics·Tanja Magoč, Steven L Salzberg
Dec 30, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marie J KiddAndrew M Collins
Jul 4, 2012·Frontiers in Immunology·John E Butler, Nancy Wertz
Nov 16, 2012·Nature·Martien A M GroenenLawrence B Schook
Jun 18, 2014·Immunogenetics·John C Schwartz, Michael P Murtaugh

❮ Previous
Next ❯

Citations

Feb 16, 2017·Annual Review of Animal Biosciences·J E ButlerMarek Sinkora
Oct 25, 2017·Immunogenetics·John C SchwartzJohn A Hammond

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Repertoire Diversity

Antibody repertoire diversity and its role during natural infection is a prerequisite for molecular and structural elucidation of functionally protective immunity. Discover the latest insights into antibody diversity here.