Genomics and proteomics of vertebrate cholesterol ester lipase (LIPA) and cholesterol 25-hydroxylase (CH25H).

3 Biotech
Roger S HolmesLaura A Cox

Abstract

Cholesterol ester lipase (LIPA; EC 3.1.1.13) and cholesterol 25-hydroxylase (CH25H; EC 1.14.99.48) play essential role in cholesterol metabolism in the body by hydrolysing cholesteryl esters and triglycerides within lysosomes (LIPA) and catalysing the formation of 25-hydroxycholesterol from cholesterol (CH25H) which acts to repress cholesterol biosynthesis. Bioinformatic methods were used to predict the amino acid sequences, structures and genomic features of several vertebrate LIPA and CH25H genes and proteins, and to examine the phylogeny of vertebrate LIPA. Amino acid sequence alignments and predicted subunit structures enabled the identification of key sequences previously reported for human LIPA and CH25H and transmembrane structures for vertebrate CH25H sequences. Vertebrate LIPA and CH25H genes were located in tandem on all vertebrate genomes examined and showed several predicted transcription factor binding sites and CpG islands located within the 5' regions of the human genes. Vertebrate LIPA genes contained nine coding exons, while all vertebrate CH25H genes were without introns. Phylogenetic analysis demonstrated the distinct nature of the vertebrate LIPA gene and protein family in comparison with other vertebrate ac...Continue Reading

References

Jun 1, 1977·The Journal of Pediatrics·A L BeaudetH S Rosenberg
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 14, 1989·Biochemistry·S J CaiL Chan
Aug 25, 1987·Biochimica Et Biophysica Acta·M W BodmerP A Lowe
Mar 27, 1987·Science·K L WionR M Lawn
Sep 1, 1994·Computer Applications in the Biosciences : CABIOS·Y Van de Peer, R De Wachter
Feb 1, 1994·European Journal of Biochemistry·D AmeisH Greten
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·R A AndersonG N Sando
Apr 7, 1999·Nature Genetics·M JayeD J Rader
May 13, 1999·The Journal of Biological Chemistry·K HirataT Quertermous
Nov 2, 2001·The Journal of Biological Chemistry·Alain RousselChristian Cambillau
Dec 6, 2002·Nature·UNKNOWN Mouse Genome Sequencing ConsortiumEric S Lander
Dec 19, 2003·Nucleic Acids Research·Jürgen Kopp, Torsten Schwede
Apr 2, 2004·Nature·Richard A GibbsUNKNOWN Rat Genome Sequencing Project Consortium
Jul 23, 2004·Journal of Biochemistry·Oliver ZschenkerDetlev Ameis
Dec 14, 2004·Nature·UNKNOWN International Chicken Genome Sequencing Consortium
Sep 2, 2005·Nature·UNKNOWN Chimpanzee Sequencing and Analysis Consortium
Sep 15, 2005·Neuroscience Letters·Nobuto ShibataEkaterina Rogaeva
Jan 10, 2006·Molecular & Cellular Proteomics : MCP·David E SleatPeter Lobel
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Serge SaxonovDouglas L Brutlag
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M Kimura
Aug 24, 2006·Genome Biology·Danielle Thierry-Mieg, Jean Thierry-Mieg
Dec 7, 2006·Nucleic Acids Research·T J P HubbardE Birney
Jan 4, 2007·The Journal of Biological Chemistry·Jennifer R DwyerFarhad Parhami
Apr 14, 2007·Science·UNKNOWN Rhesus Macaque Genome Sequencing and Analysis ConsortiumAnn S Zwieg
Apr 21, 2007·Nature Protocols·Olof EmanuelssonHenrik Nielsen
Jun 19, 2007·Trends in Ecology & Evolution·Philip C J Donoghue, Michael J Benton
Sep 18, 2008·American Journal of Physiology. Endocrinology and Metabolism·Feng WangMatti J Tikkanen
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·David R BaumanDavid W Russell
Jul 6, 2010·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Roger S HolmesJohn L VandeBerg

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Citations

May 23, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·AnggakusumaEike Steinmann
Jul 6, 2014·Journal of Medical Genetics·Marta FutemaSteve E Humphries
Jul 23, 2015·Viruses·Danielle BlondelMounira K Chelbi-Alix
Dec 22, 2016·MBio·Punya Shrivastava-RanjanChristina F Spiropoulou
Sep 1, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Farid Shokry Ataya
Mar 17, 2017·Journal of Diabetes Research·Britta NoebauerElisa Einwallner
Jan 27, 2019·Viruses·Ghada T ShawliMorgan R Herod
Dec 5, 2019·Biomolecules·Svetlana MikhailovaElena Shakhtshneider

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Software Mentioned

BLAST ( Basic Local Alignment Search Tool
AceView
BLAST
Ensembl
BLAT
Chimp
SignalP
BioEdit
GNOMON
TREECON

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