DOI: 10.1101/510586Jan 4, 2019Paper

Genomics and transcriptomics to unravel sex determination pathway and its evolution in sand flies

BioRxiv : the Preprint Server for Biology
Valeria PetrellaMarco Salvemini

Abstract

Background: Phlebotomine sand flies (Diptera, Nematocera) are important vectors of several pathogens, including Leishmania parasites, causing serious diseases of humans and dogs. Despite their importance as disease vectors, most aspects of sand fly biology remain unknown including the molecular bases of their reproduction and sex determination, aspects also relevant for the development of novel vector control strategies. Results: Using a comparative genomics/transcriptomics approach, we identified the sex determining genes in phlebotomine sand flies and proposed the first model for the sex determination cascade of these insects. For all the genes identified, we produced manually curated gene models, developmental gene expression profile and performed evolutionary molecular analysis. We identified and characterized, for the first time in a Nematocera species, the transformer (tra) homolog which exhibits both conserved and novel features. The analysis of the tra locus in sand flies and its expression pattern suggest that this gene is able to autoregulate its own splicing, as observed in the fruit fly Ceratitis capitata and several other insect species. Conclusions: Our results permit to fill the gap about sex determination in san...Continue Reading

Related Concepts

Diptera
Disease Vectors
Canis familiaris
Biological Evolution
Fruit
Gene Expression
Genes
Genetic Vectors
Insecta
Oncogenes

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