Genomics-guided discovery and structure identification of cyclic lipopeptides from the Bacillus siamensis JFL15

PloS One
Ben-Hong XuLi-Qiong Guo

Abstract

In this research, a strain with broad-spectrum antimicrobial activities was isolated from the gastrointestinal tract of hairtail (Trichiurus haumela) and identified as Bacillus siamensis JFL15 through morphological, 16S rRNA, and average nucleotide identity analyses. The genome of B. siamensis JFL15 was sequenced, and three gene clusters involved in the biosynthesis of surfactin (srf), bacillibactin (dhb), and fengycin (fen) were predicted through antiSMASH analysis. The combined genomics-metabolics profiling of the strain revealed 20 active compounds, which belong to four main types of cyclic lipopeptides produced by Bacillus species: bacillibactin, iturin, fengycin, and surfactin. Among these lipopeptides, two high-purity antifungal components, namely, components b and c, were successfully identified as iturin A and bacillomycin F. The minimum inhibitory concentrations (MICs) of iturin A for Magnapothe grisea, Rhizoctorzia solani, and Colletotrichum gloeosporioides were 125.00, 62.50, and 125.00 μg/ml, respectively, whereas the MICs of bacillomycin F for these three organisms were 62.50, 31.25, and 62.50 μg/ml, respectively. The mechanism of bacillomycin F and iturin A against M. grisea was also investigated. Scanning electro...Continue Reading

References

Apr 20, 2001·Journal of Applied Microbiology·A L MoyneS Tuzun
Jan 24, 2007·Bioinformatics·Arthur L DelcherSteven L Salzberg
Apr 25, 2007·Nucleic Acids Research·Karin LagesenDavid W Ussery
Oct 28, 2008·Nucleic Acids Research·Paul P GardnerAlex Bateman
Oct 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael Richter, Ramon Rosselló-Móra
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
Jul 21, 2012·Journal of Bacteriology·Haeyoung JeongSoo-Keun Choi
Sep 14, 2012·Organic & Biomolecular Chemistry·Martin F Kreutzer, Markus Nett
Nov 20, 2012·Applied and Environmental Microbiology·Zhihui XuRuifu Zhang
Feb 3, 2015·International Journal of Peptide Research and Therapeutics·Elżbieta KamyszWojciech Kamysz
Jul 17, 2015·Environmental Science and Pollution Research International·Ines MnifDhouha Ghribi
Aug 25, 2015·International Journal of Systematic and Evolutionary Microbiology·Christopher Dunlap
Aug 16, 2016·Journal of Industrial Microbiology & Biotechnology·Richard H Baltz

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Citations

Feb 8, 2019·Applied Microbiology and Biotechnology·Mariano A Torres MannoMartín Espariz
Jan 1, 2021·Journal of Invertebrate Pathology·Silvia Altoé FalquetoMarcos Antônio Soares
Oct 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad Fazle Rabbee, Kwang-Hyun Baek
Apr 4, 2021·Microorganisms·Mohamad Syazwan NgalimatSuriana Sabri
May 11, 2021·MicrobiologyOpen·Silvia Tenorio-SalgadoGabriel Lizama-Uc

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Datasets Mentioned

BETA
PRJNA288238

Methods Mentioned

BETA
whole-genome shotgun
size-exclusion chromatography
gel filtration
Tandem Repeat Finder
scanning electron microscopy

Software Mentioned

SE
KEGG
SOAPdenovo2
antiSMASH
JSpecies
WEGO
MAKER
rRNAmmer
EzTaxon
MEGA5

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