Genomics of estradiol-3-sulfate action in the ovine fetal hypothalamus.

Physiological Genomics
Maria Belen RabaglinoCharles E Wood

Abstract

In fetal sheep during late gestation sulfoconjugated estrogens in plasma reach a concentration 40-100 times greater than unconjugated estrogens. The objective of the present study was to determine the genomics of estradiol-3-sulfate (E(2)S) action in the ovine fetal brain. The hypothesis was that E(2)S stimulates genes involved in the neuroendocrine pathways that direct or facilitate fetal development at the end of gestation. Four sets of chronically catheterized ovine twin fetuses were studied (gestational age: 120-127 days gestation) with one infused with E(2)S intracerebroventricularly (1 mg/day) and the other remaining untreated (control). After euthanasia, mRNA samples were extracted from fetal brains. Only hypothalamic samples were employed for this study given the important function of this brain region in the control of the hypothalamus-pituitary-adrenal axis. Microarray analysis was performed following the Agilent protocol for one-color 8 × 15 microarrays, designed for Ovis aries. A total of 363 known genes were significantly upregulated by the E(2)S treatment (P < 0.05). Network and enrichment analyses were performed using the Cytoscape/Bingo software, and the results validated by quantitative real-time PCR. The main ...Continue Reading

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Citations

Oct 10, 2018·American Journal of Physiology. Endocrinology and Metabolism·Jacquelyn M WalejkoMaureen Keller-Wood
Jan 10, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Andrew AntolicMaureen Keller-Wood
Oct 28, 2014·Physiological Reports·Jarret McCartneyMaureen Keller-Wood
May 16, 2014·Physiological Genomics·Charles E WoodMaureen Keller-Wood
Jan 22, 2020·Physiological Genomics·Mitchell C LockJanna L Morrison
Sep 22, 2020·American Journal of Physiology. Endocrinology and Metabolism·Serene JosephMaureen Keller-Wood

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