PMID: 11342235May 9, 2001Paper

Genotoxic ability of cadmium, chromium and nickel salts studied by kinetochore staining in the cytokinesis-blocked micronucleus assay

Mutation Research
A I Seoane, F N Dulout

Abstract

The aneugenic and clastogenic ability of cadmium chloride(II), cadmium sulfate(II), nickel chloride(II), nickel sulfate(II), chromium chloride(III) and potassium dichromate(IV) have been evaluated through kinetochore-stained micronucleus test. Traditional genotoxicity assays evaluate DNA damage, gene mutations and chromosome breakage. However, these tests are not adequate to detect aneugenic agents that do not act directly on DNA. Staining kinetochores in the cytokinesis-blocked micronucleus assay is a useful way to discriminate between clastogens and aneuploidogens and may allow a rapid identification of aneuploidy-inducing environmental compounds. Human diploid fibroblasts (MRC-5) were employed. All compounds increased micronuclei frequency in a statistically significant way. However, increases in kinetochore-positive micronuclei frequencies were higher than in kinetochore-negative ones. The present work demonstrates the genotoxic ability of the cadmium and chromium salts studied. Aneugenic as well as clastogenic ability could be observed with this assay. Nickel salts, as it was expected because of their known weak mutagenicity, showed lower genotoxic effects than the other metal salts studied. As the test employed only allow...Continue Reading

References

Oct 1, 1992·Bulletin of Environmental Contamination and Toxicology·W R HowardT S Kochhar
Aug 1, 1992·Genome Génome / Conseil National De Recherches Canada·J H Ford, A T Correll
May 1, 1991·Environmental Health Perspectives·M Costa
Jan 1, 1991·Annual Review of Pharmacology and Toxicology·M Costa
Mar 1, 1990·Mutation Research·S De FloraP Zanacchi
May 1, 1988·Mutagenesis·F N Dulout, C C Furnus
Feb 1, 1985·Mutation Research·M Fenech, A A Morley
Jan 1, 1985·Environmental Mutagenesis·V L DellarcoR R Tice
Jul 11, 1970·Nature·J P JacobsJ P Baille
May 1, 1993·Mutation Research·M Wallin, B Hartley-Asp
Sep 1, 1994·Environmental Health Perspectives·E T Snow
Sep 1, 1994·Environmental Health Perspectives·C B KleinM Costa
Sep 1, 1994·Environmental Health Perspectives·G G FletcherE Nieboer
Apr 1, 1994·Cell Biology and Toxicology·N A LittlefieldL A Poirier
Mar 1, 1994·Molecular Carcinogenesis·L C BridgewaterS R Patierno
Mar 11, 1994·Toxicology·B el AzzouziY Thomas
Aug 1, 1996·Mutation Research·F GodetP Vasseur
Sep 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·W LiI N Chou

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Citations

Mar 30, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K DanadeviP Grover
Nov 25, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M KalafatićV Besendorfer
Jan 30, 2002·Mutation Research·Donka BenovaRobert Nilsson
Jul 3, 2013·Occupational and Environmental Medicine·Qin QianGuang Jia
May 27, 2014·Biological Trace Element Research·Ola H El-Habit, Ahmed E Abdel Moneim
Jan 1, 2012·Comparative Cytogenetics·Mikhail Vladimirovich BelousovVasily Nikolayevich Popov
Aug 7, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Victor N ZozulyaYurii P Blagoi
Oct 2, 2002·Environmental and Molecular Mutagenesis·A I SeoaneF N Dulout
Nov 17, 2009·Toxicology Letters·C DarollesF Petitot
May 15, 2007·Mutation Research·Deborah A RoubicekPatricia Ostrosky-Wegman
Jun 12, 2010·Annals of the New York Academy of Sciences·Susumu Ohshima, Atsushi Seyama
Apr 14, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·George FotakisJohn A Timbrell
Oct 21, 2010·Drug and Chemical Toxicology·Na'il SalehEtizaz Al-Kofahi
Mar 18, 2005·Genetika·V A Timoshevski, S A Nazarenko
Feb 2, 2006·Oncogene·A GlavianoC P Case
Jan 22, 2013·Toxicologic Pathology·Kristine L WittMichelle J Hooth
Jan 1, 2004·Toxicology Mechanisms and Methods·Csaba RévészIstván Gáti
Sep 29, 2005·The Journal of Bone and Joint Surgery. British Volume·A P DaviesC P Case

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