PMID: 9639703Jun 26, 1998Paper

Genotoxic activity of four inhibitors of DNA topoisomerases in larval cells of Drosophila melanogaster as measured in the wing spot assay

Mutation Research
C TorresRicardo Marcos

Abstract

Four inhibitors of DNA topoisomerases namely nalidixic acid, camptothecin, m-amsacrine and etoposide, have been evaluated for genotoxic effects in the wing spot test of Drosophila melanogaster. This assay assesses somatic recombination and mutational events. We studied nalidixic acid as an inhibitor of bacterial DNA gyrase, camptothecin as a topoisomerase I inhibitor, as well as m-amsacrine and etoposide as topoisomerse II inhibitors. The genotoxic effects were determined from the appearance of wing spots in flies trans-heterozygous for the recessive markers multiple wing hairs (mwh) and flare, as well as in flies heterozygous for mwh and the multiply inverted TM3 balancer chromosome. From our results it appears that whilst nalidixic acid and m-amsacrine were compounds that did not increase the incidence of mutant clones, camptothecin and etoposide proved to be significantly genotoxic in this test, being camptothecin more effective than etoposide. A significant proportion of the total spot induction was due to mitotic recombination, confirming previously reported data. On the other hand, the cotreatments of each topoisomerase inhibitor with the alkylating agent ethyl methanesulfonate (EMS) indicate that, while nalidixic acid, m...Continue Reading

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Citations

Oct 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·DongYu GuoHoward A Nash
Sep 12, 2007·Chemosphere·Erico R CarmonaRicardo Marcos
Jul 31, 2013·Mutation Research·Zaira da Rosa GuterresFernanda Rodrigues Garcez
Nov 3, 2010·Nanotoxicology·Eşref DemirRicardo Marcos
Apr 6, 2004·Environmental and Molecular Mutagenesis·Mostapha RizkiRicardo Marcos

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