Genotoxic and cytotoxic effects of Haas appliance in exfoliated buccal mucosa cells during orthodontic treatment.

The Angle Orthodontist
Arthur S CunhaPaulo Nelson-Filho

Abstract

To evaluate the genotoxic and cytotoxic effects of Haas appliances through micronuclei test and cytogenetic damage analysis in buccal mucosa epithelial cells of patients undergoing orthodontic treatment. Twenty-eight patients, 6-12 years of age and of both genders, who required a Haas appliance for the correction of a posterior crossbite were included. Epithelial cells from the mucosa were collected by gently scraping the inside of both the right and left cheeks. The cells were collected before the insertion of the appliance (T0), 1 month after the device was installed (T1), and again 3 months after the appliance was immobilized (T2). The cells were processed to obtain slides. Feulgen/Fast Green was used as the staining method, and the number of normal, karyolytic, pyknotic, nuclear buds, bi/trinucleated, and micronucleus cells were counted under light microscopy. Cellular abnormalities were evaluated with parametric and nonparametric tests for comparison of the means by analysis of variance testing, Tukey posttest, or the Kruskal-Wallis test and then by Dunn's posttest. The significance level was 5%. There were no statistically significant changes in the micronuclei in the evaluated periods ( P > .05). Nuclear buds increased a...Continue Reading

References

Feb 1, 1992·Mutation Research·P E TolbertJ W Allen
Mar 1, 1970·American Journal of Orthodontics·A J Haas
Aug 22, 2000·Journal of Biomedical Materials Research·J C WatahaA Schedle
Oct 13, 2001·Journal of Dental Research·H SchweiklT Spruss
Dec 11, 2003·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Fiorenzo FaccioniMaria E Fracasso
Oct 13, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Armen NersesyanSiegfried Knasmüller
Aug 22, 2007·Mutagenesis·Philip ThomasMichael Fenech
Feb 27, 2008·The Angle Orthodontist·Tatiana Siqueira GonçalvesLuciane Macedo de Menezes
Dec 11, 2008·Genetics and Molecular Research : GMR·G H WestphalenR Medina-Silva
May 16, 2009·Nature Protocols·Philip ThomasMichael Fenech
Apr 1, 2011·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Fernanda AngelieriDaniel A Ribeiro
Aug 2, 2011·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Maria P M FreitasLuciane M Menezes
Sep 6, 2011·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Hend Salah HafezYehya Ahmed Mostafa
Sep 6, 2011·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·André WeissheimerSusana Maria Deon Rizzatto
Sep 6, 2011·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Madhumitha NatarajanMalathi Narasimhan
Jun 14, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Agnieszka Machorowska-PieniążekWojciech Król
Apr 1, 2014·Journal of Materials Science. Materials in Medicine·Pía López-JornetPiedad Ramírez-Fernández
Jun 21, 2015·Environmental Toxicology and Pharmacology·Ana Martín-CameánAna M Cameán
Sep 5, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Tatiana Siqueira GonçalvesJoão Antonio Pêgas Henriques
Nov 26, 2015·Mutation Research. Reviews in Mutation Research·Claudia BolognesiMarcello Ceppi

❮ Previous
Next ❯

Citations

Mar 26, 2019·Orthodontics & Craniofacial Research·Paolo FaccioniPier Francesco Nocini
May 13, 2021·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Joao Pedro Pedrosa CruzEneida de Morais Marcílio Cerqueira

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.