Genotoxic and Cytotoxic Effects on the Immune Cells of the Freshwater Bivalve Dreissena polymorpha Exposed to the Environmental Neurotoxin BMAA

Toxins
Alexandra LepoutreEmilie Lance

Abstract

The environmental neurotoxin β-N-Methylamino-l-alanine (BMAA) has been pointed out to be involved in human neurodegenerative diseases. This molecule is known to be bioaccumulated by bivalves. However, little data about its toxic effects on freshwater mussels is available, particularly on the hemolymphatic compartment and its hemocyte cells involved in various physiological processes such as immune defenses, digestion and excretion, tissue repair, and shell production. Here we exposed Dreissena polymorpha to dissolved BMAA, at the environmental concentration of 7.5 µg of /mussel/3 days, during 21 days followed by 14 days of depuration in clear water, with the objective of assessing the BMAA presence in the hemolymphatic compartment, as well as the impact of the hemocyte cells in terms of potential cytotoxicity, immunotoxicity, and genotoxiciy. Data showed that hemocytes were in contact with BMAA. The presence of BMAA in hemolymph did not induce significant effect on hemocytes phagocytosis activity. However, significant DNA damage on hemocytes occurred during the first week (days 3 and 8) of BMAA exposure, followed by an increase of hemocyte mortality after 2 weeks of exposure. Those effects might be an indirect consequence of th...Continue Reading

References

Nov 1, 1992·JPEN. Journal of Parenteral and Enteral Nutrition·W W Souba, A J Pacitti
Jan 1, 1991·Free Radical Biology & Medicine·M K Shigenaga, B N Ames
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Jun 18, 1993·Journal of Immunological Methods·J A Smith, M J Weidemann
Nov 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Paul Alan CoxSusan J Murch
Jul 29, 2004·DNA Repair·Ari Barzilai, Ken-Ichi Yamamoto
Aug 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Susan J MurchSandra Anne Banack
Sep 10, 2004·Acta Neurologica Scandinavica·S J MurchO W Sacks
Nov 9, 2004·Current Opinion in Cell Biology·Rammohan V Rao, Dale E Bredesen
Mar 3, 2006·Environmental Science & Technology·David C AldridgeGeoff D Moggridge
May 16, 2006·Mutation Research·Izabel Vianna VillelaJoão Antonio Pêgas Henriques
Jun 22, 2007·Nature·Sheila S DavidSucharita Kundu
Mar 17, 2009·Toxicon : Official Journal of the International Society on Toxinology·Peter B Nunn, Malarvizhi Ponnusamy
Dec 16, 2009·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Doug Lobner
Jan 15, 2013·Microbial Ecology·Hans W Paerl, Timothy G Otten
Mar 20, 2013·Scientific Reports·Alexander S ChiuBrett A Neilan
Dec 19, 2013·PloS One·Estelle MasseretUNKNOWN French Network on ALS Clusters Detection and Investigation
Jan 1, 2014·Experimental Neurology·Kenneth J Rodgers
Feb 11, 2014·Ecotoxicology and Environmental Safety·Simoné DowningTimothy Grant Downing
Apr 24, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kiyo MondoDeborah C Mash
Apr 7, 2015·Toxicon : Official Journal of the International Society on Toxinology·Maranda Esterhuizen-LondtTim G Downing
Apr 24, 2015·Environmental Science and Pollution Research International·Mafalda S BaptistaVitor M Vasconcelos
Oct 16, 2015·Aquatic Toxicology·Elisabeth J FaassenMiquel Lürling
Dec 1, 2015·Toxicon : Official Journal of the International Society on Toxinology·Damien RéveillonZouher Amzil
May 4, 2016·Toxicon : Official Journal of the International Society on Toxinology·Matjaž NovakBojana Žegura
Jul 5, 2016·Fish & Shellfish Immunology·Lauris EvaristeStéphane Betoulle
Sep 7, 2016·Toxicon : Official Journal of the International Society on Toxinology·Johan RosénMatilda L Salomonsson
Sep 30, 2016·Biochemistry. Biokhimii︠a︡·A A Popova, O A Koksharova
May 5, 2017·Neurotoxicity Research·Rebecca Albano, Doug Lobner
Jun 10, 2017·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·N ChernoffW M Winnik
Dec 1, 2008·Harmful Algae·J HeislerM Suddleson

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
Flow
Assay
electrophoresis

Software Mentioned

Comet assay IV
Statsoft
Statistica

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.