Abstract
Beta-myrcene [or myrcene (1,6-Octadiene, 7-methyl-3-methylene-)] and the essential oils containing this monoterpene have been widely used in cosmetics, detergents, and soaps, and as flavoring additives for food and beverages. Due to the potentially high level of human exposure to beta-myrcene, and absence of studies involving its genotoxicity in human cells, the aim of this study was to investigate the cytotoxic and genotoxic potential of this terpenoid in non-metabolizing cells (leukocytes) and liver metabolizing cells (HepG2/C3A cells). Prior to the genotoxic assessment by the comet and micronucleus (MN) assays, a range of beta-myrcene concentrations was tested in a preliminary MTT assay. Regarding the MTT assay, the results showed cytotoxic effects for leukocytes at 250 µg/ml and higher concentrations, while for HepG2/C3A cells, absence of cytotoxicity was noted relative to all tested concentrations (after 24 hr exposure). Thus, the concentrations of 2.5, 10, 25, 50, and 100 µg/ml for leukocytes, and 2.5, 100, and 1000 µg/ml for HepG2/C3A cells were selected for subsequent assays. Genotoxicity evaluation demonstrated significant DNA damage in the comet assay and significant chromosomal abnormalities including nucleoplasmic b...Continue Reading
References
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Sep 1, 1993·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·I F DelgadoF J Paumgartten
Feb 7, 1997·Toxicology Letters·A Hartmann, G Speit
Mar 3, 1998·Toxicology·G MalichC Winder
Aug 12, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F J PaumgarttenI Chahoud
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Dec 13, 2000·Mutation Research·M Fenech
Feb 13, 2002·Journal of Neurosurgery·Rudolf Fahlbusch, Werner Schott
Jul 11, 2002·Mutation Research·Michael Fenech, Jimmy W Crott
Jul 15, 2003·Plant Physiology·Diane M MartinJörg Bohlmann
Jun 19, 2004·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·N J Hewitt, P Hewitt
Aug 23, 2005·Toxicology Letters·George Fotakis, John A Timbrell
Feb 14, 2006·Science·Eran PicherskyNatalia Dudareva
Nov 13, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F BakkaliM Idaomar
Feb 8, 2008·Journal of Hospital Medicine : an Official Publication of the Society of Hospital Medicine·John Banja, Jason S Schneider
Feb 20, 2008·Mutagenesis·Andrew R CollinsRudolf Stetina
Dec 4, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D Mitić-CulafićM Filipic
May 12, 2009·Chemico-biological Interactions·Julnar UstaShawki Dagher
Nov 23, 2010·Journal of Applied Toxicology : JAT·Fernanda PículoEdson Luis Maistro
May 4, 2011·Archives of Toxicology·Micheline Kirsch-VoldersIlse Decordier
Apr 12, 2013·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Young Woo KimByung-Mu Lee
May 1, 2013·Journal of Agricultural and Food Chemistry·Takako InuiHajime Komura
May 15, 2013·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Rosana CrespoMónica P Polo
Oct 3, 2013·Oncology Reports·Soichiro MurataKeiji Koda
Nov 18, 2014·TheScientificWorldJournal·Marianna Vieira SobralDamião Pergentino de Sousa
Oct 30, 2016·Cancer Medicine·Sanghoon LeeJu-Hong Jeon
Oct 4, 2017·Genetics and Molecular Research : GMR·P I ChaplaI Schuster
Mar 15, 2018·Journal of Toxicology and Environmental Health. Part a·María Llana-Ruiz-CabelloAna M Cameán
Mar 21, 2019·Drug and Chemical Toxicology·Ana C S SouzaEdson L Maistro