Genotoxic effects of benzo[a]pyrene and dibenzo[a,l]pyrene in a human lung cell line

International Journal of Toxicology
Silvana Andrea MourónCarlos Daniel Golijow

Abstract

Several studies have shown that polycyclic aromatic hydrocarbons (PAHs) produce genotoxic effects in assays performed in vivo and in vitro. This study was undertaken to investigate the ability of benzo[a]pyrene (BP) and dibenzo[a,l]pyrene (DBP) to induce DNA damage in a human lung fibroblast cell line (MRC-5), using sister-chromatid exchanges test (SCEs), the comet assay, and evaluating point mutations in codon 12 of the K-ras protooncogene by polymerase chain reaction-single-strand conformation polymorphisms (PCR-SSCPs) and restriction fragment length polymorphisms (RFLP)-enriched PCR methods. Sister-chromatid exchanges frequencies were significantly increased in cells exposed to benzo[a]pyrene and dibenzo[a,l]pyrene in relation to controls (p < .001). Using the standard alkaline comet assay, significant differences between groups were found for the variable comet moment (CM) when cells were exposed to BP (p < .001) and DBP (p < .001). Nevertheless, PCR-SSCP and RFLP-enriched PCR methods did not show any association between treatments with BP and DBP and K-ras point mutations. The data presented in this study indicated that BP and DBP induced both DNA strand breaks and sister-chromatid exchanges but not significant point mutat...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R L ChangA H Conney
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·D ChakravartiE G Rogan
Jun 1, 1995·International Journal of Radiation Biology·C R KentG G Steel
Aug 1, 1993·Mutation Research·P Van HummelenM Kirsch-Volders
Oct 1, 1993·Environmental Health Perspectives·H VainioT Partanen
Nov 1, 1996·Toxicology Letters·A ForniV Foà
Mar 10, 1998·Mutagenesis·D Anderson, M J Plewa
May 1, 1997·Cancer Causes & Control : CCC·P BoffettaP Gustavsson
Sep 5, 1998·Mutation Research·I KalinaR J Srám
Dec 23, 1998·Mutagenesis·G P PfeiferM Tang
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·E RojasM Valverde
Dec 29, 1999·Mutation Research·J L DurantC L Crespi
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
May 18, 2000·Journal of the National Cancer Institute·L E SmithG P Pfeifer
Jul 20, 2001·Free Radical Biology & Medicine·T S WangK Y Jan
Dec 11, 2002·Mutagenesis·A HartmannUNKNOWN 4th International Comet Assay Workshop
Jul 30, 2005·Cancer Letters·Brinda MahadevanWilliam M Baird
Oct 1, 1995·Clinical Molecular Pathology·R L WardA V Todd

❮ Previous
Next ❯

Citations

Apr 5, 2011·International Archives of Occupational and Environmental Health·Yadong WangCongke Zhang
Mar 10, 2015·The Science of the Total Environment·Yolanda Jasso-PinedaIvan Nelinho Pérez-Maldonado
Sep 25, 2014·Dose-response : a Publication of International Hormesis Society·Kenneth T Bogen
Mar 14, 2021·Environmental Pollution·Francisco Carlos da Silva JuniorSilvia Regina Batistuzzo de Medeiros

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR
chemical treatment
Assay

Software Mentioned

SPSS
Image Pro Plus

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.