Genotoxic effects of dietary and lifestyle related carcinogens in human derived hepatoma (HepG2, Hep3B) cells

Mutation Research
B J MajerS Knasmüller

Abstract

Aim of the study was to investigate the usefulness of two human derived hepatoma cell lines (HepG2 and Hep3B) for the detection of dietary and lifestyle related DNA-reactive carcinogens. Comparisons of the sensitivity of HepG2 cells of different origin towards benzo[a]pyrene (B(a)P) showed that strong differences exist in the induction of micronuclei (MN). The most sensitive was used for all further experiments, in which we investigated the effects of aflatoxin B(1) (AFB(1)), B(a)P, As(2)O(3), CdCl(2), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), N-nitrosodimethylamine (NDMA), N-nitrosopyrrolidine (NPYR), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), ethanol, acetaldehyde and caffeic acid in micronucleus (MN) tests. Dose dependent effects were detected in HepG2 with AFB(1) (0.2microM), CdCl(2) (2.2microM), As(2)O(3) (8.1microM), B(a)P (22.7microM), PhIP (35.7microM), NDMA (22.7mM), acetaldehyde (11.2mM) and ethanol (442.2mM). Numbers in parentheses indicate the C(D) values (concentration that induced a two-fold increase over the background). NNK and caffeic acid gave negative results under all conditions. In Hep3B cells, the effects were generally weaker. With PhIP, As(2)O(3) and NDMA negative results were ob...Continue Reading

References

Dec 1, 1992·Carcinogenesis·W K Lutz, J Schlatter
Sep 1, 1989·Mutation Research·J D TuckerJ S Felton
Jan 1, 1988·Mutation Research·M D Shelby
Dec 19, 1973·Biochimica Et Biophysica Acta·A Foldes, J L Meek
Apr 11, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·R J Anderson, R M Weinshilboum
Dec 8, 1980·Life Sciences·D Y Lai, J C Arcos
Aug 1, 1995·Archives of Environmental Contamination and Toxicology·J C CodinaA de Vicente
Jul 26, 2000·Teratogenesis, Carcinogenesis, and Mutagenesis·M P DuarteJ Rueff
Mar 7, 2001·Mutagenesis·B J Phillips, P Jenkinson
Aug 4, 2001·Toxicology and Applied Pharmacology·L M Del RazoD J Thomas
Apr 25, 2002·Mutagenesis·Veronika EhrlichSiegfried Knasmueller
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T FaturM Filipic
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B LakyF Kassie
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·V EhrlichS Knasmüller
Nov 12, 2002·Environmental Health Perspectives·Miroslav StýbloDavid J Thomas
Mar 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·B J MajerS Knasmüller

❮ Previous
Next ❯

Citations

Dec 14, 2011·Environmental Science and Pollution Research International·Manuel Garcia-KäuferVolker Mersch-Sundermann
Mar 26, 2013·Biomaterials·Scott H MedinaMohamed E H Elsayed
Aug 20, 2009·The Journal of Biological Chemistry·Kari N Nejak-BowenSatdarshan P Monga
Oct 29, 2010·Journal of Occupational Medicine and Toxicology·Céline GasnierGilles-Eric Séralini
Mar 23, 2011·Arhiv za higijenu rada i toksikologiju·Ivan KosalecNevenka Kopjar
Aug 3, 2012·Journal of Toxicology and Environmental Health. Part a·G A R OliveiraD J Dorta
Apr 30, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kathleen BoehmeStefan O Mueller
Dec 18, 2010·Mutagenesis·M MisíkS Knasmueller
Nov 8, 2011·Mutagenesis·Rozenn JosséAndré Guillouzo
Sep 19, 2008·Expert Opinion on Drug Metabolism & Toxicology·André Guillouzo, Christiane Guguen-Guillouzo
Mar 21, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Rone Aparecido De GrandisEliana Aparecida Varanda
Jul 6, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jelena KaticIlse Decordier
Mar 24, 2010·Toxicology and Applied Pharmacology·Julie DumontAndré Guillouzo
Aug 31, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M S TsuboyL R Ribeiro
Apr 27, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Janja PlazarGeny M M Groothuis
Oct 10, 2013·Environmental and Molecular Mutagenesis·Laure KhouryMarc Audebert
Feb 1, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B J MajerS Knasmüller
Jul 1, 2004·Mutation Research·Andrew R Collins, Lynnette R Ferguson
Jun 27, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Loganathan GayathriMohammad A Akbarsha
Dec 6, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Olga ViegasMetka Filipič
Jul 28, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Takeshi MoritaLeonard Schechtman
Dec 4, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Aline M PrietoChristiane P Soares
Apr 19, 2018·Environmental and Molecular Mutagenesis·Stephanie L Smith-RoeKristine L Witt
Oct 20, 2007·Environmental and Molecular Mutagenesis·Guo-Dong ZhouKurt Randerath
Mar 19, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Rozenn JosséAndré Guillouzo
Oct 28, 2017·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Emilie SoutonChagnon Marie-Christine
Jul 10, 2020·Genetics and Molecular Biology·Lívia Cristina HernandesLusânia Maria Greggi Antunes
Nov 10, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D DesaulniersC Parfett

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.