Genotoxic effects of PCB 52 and PCB 77 on cultured human peripheral lymphocytes

Mutation Research
Suleyman SandalDavid O Carpenter

Abstract

Polychlorinated biphenyls (PCBs) are known to be carcinogenic, but the mechanisms of this action are uncertain. Most, but not all, studies have concluded that PCBs are not directly mutagenic, and that much if not all of the carcinogenic activity resides in the fraction of the PCB mixture that contains congeners with dioxin-like activity. The present study was designed to determine genotoxic effects of an ortho-substituted, non-coplanar congener, 2,2',5,5'-tetrachlorobiphenyl (PCB 52), and a non-ortho-substituted coplanar congener with dioxin-like activity, 3,3',4,4'-tetrachlorobiphenyl (PCB 77) on cultured human peripheral lymphocytes. DNA damage was assessed by use of the comet assay (alkaline single-cell gel electrophoresis). After cell cultures were prepared, test groups were treated with different concentrations of PCB 52 (0.2 and 1 microM) and PCB 77 (1 and 10 microM) for 1h at 37 degrees C in a humidified carbon dioxide incubator, and compared to a DMSO vehicle control group. The cells were visually classified into four categories on the basis of extent of migration such as undamaged (UD), low damage (LD), moderate damage (MD) and high damage (HD). The highest concentration of PCBs 52 and 77 significantly increased DNA br...Continue Reading

References

Jan 1, 1990·Free Radical Biology & Medicine·S J Stohs
Sep 1, 1989·Mutation Research·L SargentL Meisner
Jul 1, 1996·Mutagenesis·K BelpaemeM Kirsch-Volders
Apr 16, 1998·Environmental Health Perspectives·L G Hansen
Jun 10, 1998·Environmental Health Perspectives·V J Cogliano
Nov 30, 1998·Environmental Health Perspectives·M Van den BergT Zacharewski
Feb 14, 2003·Toxicology and Applied Pharmacology·Parvaneh EspandiariLarry W Robertson
Mar 26, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Adedayo AdeekoBarbara F Hales
Sep 25, 2004·Ecotoxicology and Environmental Safety·Gilbert Ross
Dec 16, 2004·Environmental Health Perspectives·Chad M VezinaJames R Olson
Oct 20, 2006·Critical Reviews in Toxicology·Stefanie Knerr, Dieter Schrenk
Apr 1, 1972·Environmental Health Perspectives·D B PeakallS E Bloom

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Citations

Dec 6, 2012·Cancer Letters·Gabriele Ludewig, Larry W Robertson
May 28, 2010·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Niyazi TugBayram Yilmaz
Nov 1, 2013·Drug and Chemical Toxicology·Ufuk ErenberkUlkan Kilic
Nov 28, 2014·Environmental Science and Pollution Research International·Basem Shomar, Anne Dare
Mar 31, 2015·Reviews on Environmental Health·David O Carpenter
Jan 11, 2018·Archives of Environmental Contamination and Toxicology·Davor ŽelježićJudita Grzunov Letinić
Apr 7, 2011·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Chi Y HsiehBo Y Yu
Mar 14, 2018·Environmental Science and Pollution Research International·Shixiang DaiWenjie Ren
Mar 25, 2020·Dose-response : a Publication of International Hormesis Society·Ainy ZehraZaigham Abbas
Nov 20, 2018·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Laurentius Ron Hoogenboom
Aug 19, 2020·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Melek Ece ÖngelMustafa Özilgen
Jun 26, 2021·Environmental Toxicology and Pharmacology·Maria-Sofia VidaliPanagiotis Georgiadis

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