Genotoxic effects of p,p'-DDT (1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane) and its metabolites in Zebra mussel (D. polymorpha) by SCGE assay and micronucleus test

Environmental and Molecular Mutagenesis
Andrea BinelliAlfredo Provini

Abstract

This is the first study to evaluate the potential genotoxicity of p,p'-DDT (1,1,1-trichloro-2,2-bis-(chlorophenyl)ethane) and its metabolites (p,p'-DDD and p,p'-DDE) in the sentinel mollusc Zebra mussel (Dreissena polymorpha). DNA damage was measured using the single cell gel electrophoresis (SCGE) assay and the micronucleus test (MN test), which represent two of the more sensitive biomarkers for genotoxicity evaluation. Three different concentrations (0.1, 2, and 10 mug/L) of each compound were administered in water for 168 hr, maintaining mussels at constant laboratory conditions and collecting several specimens every 48 hr for biochemical analyses. At the same time, the bioaccumulation process and the concentration/effect relationship were checked by GC-MS/MS analyses of mussel soft tissues. The SCGE assay results showed a clear and significant (P < 0.05) relationship between DNA injuries and tested doses for all the homologues throughout the 7-day exposure period. The final DNA damage due to p,p'-DDE was almost double that of the other two homologues that showed the same toxicity pattern. The micronucleus frequency analysis confirmed the genotoxicity potential of the three homologues and p,p'-DDE showed the highest irrevers...Continue Reading

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Citations

Jul 28, 2011·Environmental Science and Pollution Research International·Bernhard WetterauerHenner Hollert
May 11, 2011·International Journal of Environmental Research and Public Health·Alejandro Canales-AguirreRuth De Celis
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Jul 26, 2019·Environmental Toxicology and Chemistry·Eirik D NeerlandÅse Krøkje
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Jan 12, 2021·Ecotoxicology and Environmental Safety·Helene InderbergÅse Krøkje
Aug 30, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Melissa FariaCarlos Barata

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