Genotoxic evaluation of chloroacetonitrile in murine marrow cells and effects on DNA damage repair gene expressions

Mutagenesis
Sabry M AttiaO A Al-Shabanah

Abstract

Although chloroacetonitrile (CAN), a disinfection by-product of chlorination of drinking water, is considered a rodent carcinogen that induces lung adenomas in mice, previous studies on its genotoxicity have yielded inconclusive results. Thus, its cancer mode of action has not been clearly defined. We evaluated CAN-induced genotoxicity in mice using mouse bone marrow micronucleus test, comet assays and expression of genes associated with DNA damage repair. Mice exposed to CAN at 8.75, 17.5, 35 and 52.5mg/kg for 7 days did not exhibit any significant increases in the incidence of micronuclei formation at 24 and 48h after last exposure. However, CAN caused significant suppressions of erythroblast proliferation at the highest dose. In the alkaline comet assay, there was a significant increase in the incidence of DNA strand breaks in mice killed after 3h of last treatment with 35 and 52.5mg/kg/day CAN, while no significant difference in the DNA strand breaks was found in mice killed after 24h of the last treatment. However, slight (but significant) CAN-induced oxidative DNA damage was detected following Fpg digestion at 3-h sampling time, digestion with EndoIII resulted in considerable increases in oxidative DNA damage at 3 and 24h...Continue Reading

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Citations

Sep 25, 2015·Mutagenesis·Sabry M AttiaSaleh A Bakheet
Nov 3, 2016·PloS One·Aroldo Vieira de Moraes FilhoLee Chen-Chen
Jun 16, 2016·Journal of Biochemical and Molecular Toxicology·Saleh A BakheetSabry M Attia
Nov 17, 2020·The Science of the Total Environment·Chang Gyun ParkYoung Jun Kim
Apr 6, 2020·Toxicology Letters·Andrew CollinsAmaya Azqueta
Feb 9, 2020·Environmental Science & Technology·Huiyuan ZhaoRugang Zhong

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