Genotoxic potential of TiO2 on bottlenose dolphin leukocytes

Analytical and Bioanalytical Chemistry
Margherita BernardeschiM Nigro

Abstract

Titanium dioxide is extensively used in a variety of products, including industrial materials and cosmetics. Studies mainly performed on human cell lines and in vivo exposure on experimental animals have raised concern about the toxic effects of ultrafine titanium dioxide; however, scarce information is available about its impact on aquatic life. The aim of this article was to assess the genotoxic potential of TiO(2) (anatase and rutile) on bottlenose dolphin leukocytes. Blood samples were obtained from four male and one female specimens reared at the Adriatic SeaWorld "Oltremare" (Riccione, Italy). Leukocytes were isolated by the lyses procedure and in vitro exposed to TiO(2) in RPMI. Experimental solutions were sonicated immediately before dosing the cells. Three exposure times (4, 24 and 48 h) and three doses (20, 50 and 100 microg/ml) were tested. Genotoxicity was detected by the single-cell gel electrophoresis (or comet assay) at pH > or = 13, assessing single/double-strand breaks and alkali-labile sites. Cytotoxicity was also detected by the Trypan blue exclusion method. Results showed that both the crystalline forms of TiO(2) were genotoxic for bottlenose dolphin leukocytes, with a statistically significant increase of D...Continue Reading

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Citations

Oct 22, 2013·Marine Pollution Bulletin·Richard F LeePatricia A Fair
Jul 20, 2010·Analytical and Bioanalytical Chemistry·Hanna L Karlsson
Mar 26, 2013·Analytical and Bioanalytical Chemistry·K SunjogM Lenhardt
Mar 29, 2014·Journal of Food and Drug Analysis·Tao ChenYan Li
Feb 11, 2014·Environment International·D MinettoA Volpi Ghirardini
Oct 24, 2014·Environmental Science and Pollution Research International·Yurong WangQingdai Liu
Oct 11, 2017·Nanoscale·Christopher M SimsBryant C Nelson

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