Genotoxic testing of titanium dioxide anatase nanoparticles using the wing-spot test and the comet assay in Drosophila

Mutation Research. Genetic Toxicology and Environmental Mutagenesis
Erico R CarmonaRicard Marcos

Abstract

Titanium dioxide nanoparticles (TiO2 NPs) are widely used for preparations of sunscreens, cosmetics, food and personal care products. However, the possible genotoxic risk associated with this nano-scale material exposure is not clear, especially in whole organisms. In the present study, we explored the in vivo genotoxic activity of TiO2 NPs as well as their TiO2 bulk form using two well-established genotoxic assays, the wing spot test and the comet assay in Drosophila melanogaster. To determine the extent of tissue damage induced by TiO2 NPs in Drosophila larvae, the trypan blue dye exclusion test was also applied. Both compounds were supplied to third instar larvae by ingestion at concentration ranging from 0.08 to 1.60 mg/mL. The results obtained in the present study indicate that TiO2 NPs can reach and induce cytotoxic effects on midgut and imaginal disc tissues of larvae, but they do not promote genotoxicity in the wing-spot test of Drosophila. However, when both nano- and large-size forms of TiO2 were evaluated with the comet assay in Drosophila hemocytes, a significant increase in DNA damage, with a direct dose-response pattern, was observed for TiO2 NPs. The results obtained with the comet assay suggest that the primary ...Continue Reading

References

Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
May 1, 1970·Mutation Research·M A Kastenbaum, K O Bowman
Jan 1, 1994·Critical Reviews in Toxicology·C Sengstag
Sep 5, 1998·Mutation Research·U GrafF E Würgler
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Sep 26, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·R J Aitken, C Krausz
May 16, 2006·Mutation Research·Izabel Vianna VillelaJoão Antonio Pêgas Henriques
Oct 4, 2006·Neuron·J Lawrence Marsh, Leslie Michels Thompson
Apr 22, 2009·Toxicology Letters·Vyom SharmaAlok Dhawan
May 12, 2009·Biomaterials·Neenu SinghShareen H Doak
Oct 9, 2009·The New England Journal of Medicine·Jan H J Hoeijmakers
Oct 31, 2009·Toxicology and Applied Pharmacology·Maqusood AhamedJohn J Rowe
Nov 6, 2009·Cancer Research·Benedicte TrouillerRobert H Schiestl
Jan 29, 2010·Toxicology·Kai SavolainenGerhard Kasper
Jul 24, 2010·Analytical and Bioanalytical Chemistry·Alok Dhawan, Vyom Sharma
Aug 27, 2010·Environmental and Molecular Mutagenesis·Erico R CarmonaRicardo Marcos
Nov 3, 2010·Nanotoxicology·Eşref DemirRicardo Marcos
Nov 26, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ritesh K ShuklaAlok Dhawan
Jun 8, 2011·Mutation Research·Erico R CarmonaRicard Marcos
Oct 19, 2011·Toxicology and Applied Pharmacology·Nicola A PhilbrookVirginia K Walker
Oct 22, 2011·Journal of Applied Toxicology : JAT·Katiane Cella GabrielHeloisa Helena Rodrigues de Andrade
Nov 19, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Giuseppe VecchioPier P Pompa
Dec 27, 2011·Regulatory Toxicology and Pharmacology : RTP·Masato NayaJunko Nakanishi
Jan 21, 2012·Environmental Science & Technology·Alex WeirNatalie von Goetz
May 3, 2012·Nanotoxicology·Gerard ValesRicard Marcos
May 11, 2012·Journal of Clinical Biochemistry and Nutrition·Ming-Fen SongKazuaki Kawai
Jun 30, 2012·Journal of Applied Toxicology : JAT·Robert S WoodruffTao Chen
Mar 28, 2013·Nanoscale·Liang-Chien ChengRu-Shi Liu
Apr 17, 2013·Particle and Fibre Toxicology·Hongbo ShiJinshun Zhao
May 21, 2013·Archives of Toxicology·Amaya Azqueta, Andrew R Collins

❮ Previous
Next ❯

Citations

Feb 26, 2016·International Journal of Molecular Sciences·Mariana Carmen ChifiriucAlexandru Al Ecovoiu
Sep 24, 2015·Ecotoxicology and Environmental Safety·Abdelmonem M Khalil
Apr 3, 2016·Mutation Research. Reviews in Mutation Research·Maria AugustyniakMarta Dziewięcka
Sep 5, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Erico R CarmonaRicard Marcos
Apr 30, 2016·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Mohamed AlarabyAlba Hernández
Aug 27, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Érica de Melo ReisMário Antônio Spanó
Feb 25, 2017·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Ronan MaguireKevin Kavanagh
Jun 24, 2017·Journal of Natural Medicines·Pornsiri PitchakarnPornngarm Limtrakul
Dec 28, 2018·Nanotoxicology·Bedanta Kumar Barik, Monalisa Mishra
Sep 3, 2020·Toxicology and Industrial Health·Burhan Nas, Deniz Altun Çolak
Sep 24, 2020·Toxicology and Industrial Health·Mohammad Rafiq Wani, Ggha Shadab
May 13, 2019·Environmental Science and Pollution Research International·Subhashree PriyadarsiniMonalisa Mishra
Nov 2, 2019·The Journal of Experimental Biology·Jan LubawyHervé Colinet
Dec 31, 2020·Journal of Toxicology and Environmental Health. Part a·Orawan KhantamatPiya Temviriyanukul
Feb 23, 2021·Beilstein Journal of Nanotechnology·Sandra Senyo FometuJoan Shine Davids
Sep 28, 2020·International Journal of Biological Macromolecules·Janmejaya BagMonalisa Mishra

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.