Genotoxicity and mutagenicity of melanoidins isolated from a roasted glucose-glycine model in human lymphocyte cultures, intestinal Caco-2 cells and in the Salmonella typhimurium strains TA98 and TA102 applying the AMES test

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Sigrid GlöslIbrahim Elmadfa

Abstract

Melanoidins are formed during household cooking procedures and are part of our daily diet, but data on their toxicological potential are still scarce. Therefore, the mutagenic, cytotoxic and genotoxic activity of the water soluble total fraction (sol A), the water soluble high molecular weight fraction (HMW; Molecular weight>12,400 Da) and the remaining water soluble low molecular weight fraction (LMW) isolated from a glucose-glycine model system roasted at 125 degrees C was comprehensively studied in human lymphocytes (genetic end point: sister chromatid exchange (SCE)), Caco-2 cells (SCE, cell viability, cell proliferation) and in the Salmonella typhimurium strains TA98 and TA102 (Ames test). Tests were performed in a dose- and time-dependent manner. The results indicate a significant increase in SCE formation in human lymphocytes after the exposure to 0.05% and 0.1% of the melanoidin fractions. In Caco-2 cells, only the exposure to LMW increased the SCE formation as a matter of concentration. Cell's proliferation and viability decreased significantly after exposure to melanoidins. In the AMES test, melanoidins did not show a mutagenic potential, neither using the TA98 nor the TA102 strain. These results show that melanoidins...Continue Reading

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Citations

Jan 15, 2016·International Journal of Biological Macromolecules·Jianjun ZhangLe Jia
Sep 26, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Abdulaziz Qaid AliAb Rani Samsudin
Mar 29, 2007·Molecular Nutrition & Food Research·Karl-Heinz WagnerCatherine Billaud
Mar 31, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yixing LiWeihong Ma
Apr 13, 2018·Journal of the Science of Food and Agriculture·Chih-Yuan KoSzu-Chuan Shen
May 16, 2012·Food & Function·Ana S P MoreiraManuel A Coimbra
Jun 1, 2005·Molecular Nutrition & Food Research·Veronika Somoza
Jan 23, 2020·Nutrients·Nesreen AljahdaliFranck Carbonero
Mar 2, 2021·Food Research International·Carina Quintanilha da SilvaElisa Raquel Anastácio Ferraz
Nov 1, 2011·Trends in Food Science & Technology·Tomaz LangerholcAvrelija Cencic

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