PMID: 9637241Jun 24, 1998Paper

Genotoxicity and mutagenicity of the alpha, beta-unsaturated carbonyl compound crotonaldehyde (butenal) on a plasmid shuttle vector

Mutation Research
C CzernyT M Rünger

Abstract

Crotonaldehyde is an alpha,beta-unsaturated carbonyl compound and an important environmental and industrial toxic substance. Its mutagenic and carcinogenic properties are related to its reactivity to DNA, where it forms different guanine adducts. In order to study the mutagenic consequences of this agent in intact human cells, we treated the shuttle vector plasmid pZ189 with different doses of crotonaldehyde at 37 degrees C for 2 h and then transfected the such damaged plasmid into the normal human lymphoblast cell line GM0621. Within these host cells the guanine adducts are repaired and the plasmids replicated by cellular enzymes. After 2.5 days replicated plasmids were purified from the cells and plasmid survival was quantitated by transformation ability. With increasing doses of crotonaldehyde, we found a significant decline of plasmid survival, reflecting a pronounced genotoxicity of crotonaldehyde-induced DNA damage in intact human cells. Using the plasmid encoded mutagenesis marker gene supF, we were able to screen for mutants and determine mutation frequency in recovered plasmids. A significant increase in mutation frequency with increasing doses of crotonaldehyde reflects mutagenicity of crotonaldehyde-induced DNA damag...Continue Reading

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Citations

Feb 13, 2014·International Journal of Biological Macromolecules·Badar Ul IslamAsif Ali
Jun 6, 2000·Mutation Research·K A Canella, M M Seidman
Feb 4, 2005·Environmental and Molecular Mutagenesis·Priscilla H FernandesR Stephen Lloyd
Jun 26, 2001·Environmental and Molecular Mutagenesis·E Eder, C Deininger
Sep 17, 2011·Chemistry & Biodiversity·Michael P StoneGanesh Shanmugam
Mar 20, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Shigeyuki KitamuraShigeru Ohta

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